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| Status |
Public on Jan 09, 2014 |
| Title |
Identification of IL-21-induced STAT3 dependent genes in human B cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3
We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner
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| Overall design |
Sort-purified naïve B cells from four healthy donors and three patients with AD-HIES due to heterozygous STAT3 mutations were cultured for four days with CD40L in the presence or absence of IL-21 (50ng/ml). After four days, RNA was extracted from the harvested cells and the genetic profile was analysed by microarray.
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| |
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| Contributor(s) |
Berglund L, Tangye S |
| Citation(s) |
24159173 |
| |
| Submission date |
Oct 23, 2013 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Stuart Tangye |
| E-mail(s) |
s.tangye@garvan.org.au
|
| Organization name |
Garvan Institute for Medical Research
|
| Department |
Immunology
|
| Street address |
384 Victoria St
|
| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA223638 |
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