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Series GSE50812 Query DataSets for GSE50812
Status Public on Sep 13, 2013
Title Aiolos promotes anchorage independence by silencing p66Shc transcription in lung carcinoma A549 cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Anchorage of tissue cells to their physical environment is an obligate requirement for survival that is lost in mature hematopoietic and in transformed epithelial cells. Here we find that a lymphocyte lineage-restricted transcription factor, Aiolos, is frequently expressed in lung cancers and predicts markedly reduced patient survival. Aiolos decreases expression of a large set of adhesion-related genes, disrupting cell-cell and cell-matrix interactions. Aiolos also reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66Shc and blocking anoikis in vitro and in vivo. In lung cancer tissues and single cells, p66Shc expression inversely correlates with that of Aiolos. Together, these findings suggest that Aiolos functions as an epigenetic driver of lymphocyte mimicry in metastatic epithelial cancers.
Overall design Two-condition experiment, A549-empty vs. A549-Aiolos cells. Biological replicates: 3 control, 3 transfected, independently grown and harvested. One replicate per array.
Contributor(s) Liu Z, Terada LS, Li X, Xu Z, Du W, Ma Z, Yao Z, Wei Y, Zhang Z, Wang H, Sun B, Zhao X, Wang L, Minna JD, Zhu Z, Gong Y, Niu Q, Qin L, Girard L
Citation(s) 24823637
Submission date Sep 12, 2013
Last update date Feb 21, 2021
Contact name Zhenyi Ma
Phone 86-22-83336533
Organization name Tianjin Medical University
Department Biochemistry and Molecular Biology
Street address 22 Qixiangtai Road
City Tianjin
ZIP/Postal code 300070
Country China
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (3)
GSM1230233 Aiolos-overexpressing A549 cells Replicate 1
GSM1230234 Aiolos-overexpressing A549 cells Replicate 2
GSM1230235 Aiolos-overexpressing A549 cells Replicate 3
BioProject PRJNA219064

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Supplementary file Size Download File type/resource
GSE50812_RAW.tar 64.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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