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| Status |
Public on Sep 13, 2013 |
| Title |
Aiolos promotes anchorage independence by silencing p66Shc transcription in lung carcinoma A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Anchorage of tissue cells to their physical environment is an obligate requirement for survival that is lost in mature hematopoietic and in transformed epithelial cells. Here we find that a lymphocyte lineage-restricted transcription factor, Aiolos, is frequently expressed in lung cancers and predicts markedly reduced patient survival. Aiolos decreases expression of a large set of adhesion-related genes, disrupting cell-cell and cell-matrix interactions. Aiolos also reconfigures chromatin structure within the SHC1 gene, causing isoform-specific silencing of the anchorage reporter p66Shc and blocking anoikis in vitro and in vivo. In lung cancer tissues and single cells, p66Shc expression inversely correlates with that of Aiolos. Together, these findings suggest that Aiolos functions as an epigenetic driver of lymphocyte mimicry in metastatic epithelial cancers.
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| Overall design |
Two-condition experiment, A549-empty vs. A549-Aiolos cells. Biological replicates: 3 control, 3 transfected, independently grown and harvested. One replicate per array.
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| Contributor(s) |
Liu Z, Terada LS, Li X, Xu Z, Du W, Ma Z, Yao Z, Wei Y, Zhang Z, Wang H, Sun B, Zhao X, Wang L, Minna JD, Zhu Z, Gong Y, Niu Q, Qin L, Girard L |
| Citation(s) |
24823637 |
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| Submission date |
Sep 12, 2013 |
| Last update date |
Feb 21, 2021 |
| Contact name |
Zhenyi Ma |
| E-mail(s) |
zhyma@tmu.edu.cn
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| Phone |
86-22-83336533
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| Organization name |
Tianjin Medical University
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| Department |
Biochemistry and Molecular Biology
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| Street address |
22 Qixiangtai Road
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| City |
Tianjin |
| ZIP/Postal code |
300070 |
| Country |
China |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (3) |
| GSM1230233 |
Aiolos-overexpressing A549 cells Replicate 1 |
| GSM1230234 |
Aiolos-overexpressing A549 cells Replicate 2 |
| GSM1230235 |
Aiolos-overexpressing A549 cells Replicate 3 |
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| Relations |
| BioProject |
PRJNA219064 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50812_RAW.tar |
64.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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