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Series GSE50805 Query DataSets for GSE50805
Status Public on Dec 01, 2013
Title The effect of MPO inhibitor on the gene expression profiles in HL-60 cell lines exposed to 1, 2, 4,-benzenetriol
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To reveal the gene expression profile changes after exposure to BT, one of benzene metabolites, with or without ABAH, a myeloperoxidase (MPO) inhibitor, and to elucidate how MPO is involved in myelotoxicity of BT, we performed microarray analysis. HL-60 cells suspended in RPMI 1640 containing with 10% of heat-inactivated FBS at 4×10^5/ mL were incubated with or without BT (50 μM) at 37℃ for one or 4 hours. For MPO inhibition experiment, HL-60 (4×10^5 cells/ mL) were pretreatment with 100 μM of ABAH in RPMI 1640/10% of heat-inactivated FBS at 37℃ for 24 hours. Media was then replaced with new media containing the regent plus BT (50 μM) and incubate at 37℃ for one or 4 hours. Unexposed HL-60 cells were used as controls. To validate the microarray results, we selected 22 genes and conducted a real-time PCR.
Overall design Gene expression alteration induced by 50 μM of BT in HL-60 cells, with or without ABAH, was measured at 1 and 4 hours after exposure to it. Three independent experiments were performed at each time (1 or 4 hours).
Contributor(s) Nishikawa T, Miyahara E
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Submission date Sep 12, 2013
Last update date Jan 09, 2018
Contact name Takuro Nishikawa
Organization name Kagoshima University
Department Pediatrics
Street address Sakuragaoka 8-35-1
City Kagoshima
ZIP/Postal code 890-8520
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (8)
GSM1229959 Control_1h
GSM1229960 BT_1h
GSM1229961 ABAH_1h
BioProject PRJNA219057

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50805_RAW.tar 98.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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