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| Status |
Public on Dec 01, 2013 |
| Title |
The effect of MPO inhibitor on the gene expression profiles in HL-60 cell lines exposed to 1, 2, 4,-benzenetriol |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To reveal the gene expression profile changes after exposure to BT, one of benzene metabolites, with or without ABAH, a myeloperoxidase (MPO) inhibitor, and to elucidate how MPO is involved in myelotoxicity of BT, we performed microarray analysis. HL-60 cells suspended in RPMI 1640 containing with 10% of heat-inactivated FBS at 4×10^5/ mL were incubated with or without BT (50 μM) at 37℃ for one or 4 hours. For MPO inhibition experiment, HL-60 (4×10^5 cells/ mL) were pretreatment with 100 μM of ABAH in RPMI 1640/10% of heat-inactivated FBS at 37℃ for 24 hours. Media was then replaced with new media containing the regent plus BT (50 μM) and incubate at 37℃ for one or 4 hours. Unexposed HL-60 cells were used as controls. To validate the microarray results, we selected 22 genes and conducted a real-time PCR.
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| Overall design |
Gene expression alteration induced by 50 μM of BT in HL-60 cells, with or without ABAH, was measured at 1 and 4 hours after exposure to it. Three independent experiments were performed at each time (1 or 4 hours).
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| Contributor(s) |
Nishikawa T, Miyahara E |
| Citation missing |
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| Submission date |
Sep 12, 2013 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Takuro Nishikawa |
| E-mail(s) |
adu44150@ams.odn.ne.jp
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| Organization name |
Kagoshima University
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| Department |
Pediatrics
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| Street address |
Sakuragaoka 8-35-1
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| City |
Kagoshima |
| ZIP/Postal code |
890-8520 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA219057 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50805_RAW.tar |
98.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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