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| Status |
Public on Apr 18, 2014 |
| Title |
Expression data from C. elegans during L4, L4-lethargus, and Adult |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
To identify genes differentially expressed during the molt, we collected RNA 30-40 minutes after feeding cessation at the start of the fourth larval stage (L4) lethargus. Additional time points for RNA collection were in the mid-L4 stage, approximately four hours prior to lethargus, and in the young adult stage, four hours after lethargus. These samples were interrogated with the Affymetrix C. elegans Genome Array.
A total of 1,804 gene transcripts were up regulated, and 1,088 gene transcripts were down regulated, during the L4 lethargus period compared to the L4 and Adult stages (false discovery rate (FDR) < 0.05).
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| Overall design |
There were a total of 3 groups and 5x replication for each group, for 15 total samples that were analyzed. The groups were (1) L4, (2) L4-lethargus, (3) Adult. We generated the following pairwise comparisons using R/maanova: L4-lethargus vs L4, L4-lethargus vs Adult. The permutation based p-values for each test were multiplied by 2 and transcripts with an FDR≤5% were selected.
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| Contributor(s) |
Shockley KR, Raizen DM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 22, 2013 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Keith Shockley |
| Organization name |
NIEHS
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| Street address |
111 TW Alexander Drive
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA198532 |
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