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Series GSE45718 Query DataSets for GSE45718
Status Public on Mar 30, 2014
Title Expression data from early and late vegetative stages from rice hybrid and its parents
Platform organism Oryza sativa
Sample organism Oryza sativa Indica Group
Experiment type Expression profiling by array
Summary Heterosis is a complex phenomenon governed by many phenotypes in combination. In comparative account of the different stages a better understanding regulating this phenomenon in terms of gene action can be ascertained.
We used microarrays to ascertained the kind and extent of gene actions in the different developmental stages to reach to the conclusion of contribution of different mode of gene actions.
 
Overall design A total of 45 arrays representing in triplicates of each five developmental stages of an Indica hybrid and its parents five vegetative developmental stages. Indica hybrid (MRP5401) has been take along with its parents (female parent PMS80 (B Line) and male parent PR319 (R line)) and five stages are selected for rna extraction and hybridization on Affymetirx microarray comprising young leaf (Leaves of 14-days-old seedling), young root (Roots of 14-days-old seedlings), Leaves of 40-60-days-old plants, Roots of 40-60-days-old plants and Flag Leaves of 90-days-old plants. Further these stages are named accordingly as YL_B, YL_H, YL_R (Leaves of 14-days-old seedlings of B parent, hybrid and R parent), YR_B, YR_H, YR_R (Roots of 14-days-old seedlings of B parent, hybrid and R parent), ML_B, ML_H, ML_R (Leaves of 40-60-days-old plants of B parent, hybrid and R parent), BMR, HMR, RMR (Roots of 40-60-days-old plants of B parent, hybrid and R parent) and BFL, HFL, RFL (Flag leaves of 90-days-old B parent, hybrid and R parent).
Development course
 
Contributor(s) Baranwal VK, Manupriya M, Umate P, Mikkilineni V, Zehr UB, Tyagi AK, Kapoor S
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Submission date Apr 02, 2013
Last update date Dec 27, 2017
Contact name Sanjay Kapoor
E-mail(s) kapoors@genomeindia.org
Phone 91 11 65171135
URL http://www.dpmb.ac.in/
Organization name University of Delhi South Campus
Department Department of Plant Molecular Biology
Street address Benito Juarez Road
City New Delhi
State/province Delhi
ZIP/Postal code 110021
Country India
 
Platforms (1)
GPL2025 [Rice] Affymetrix Rice Genome Array
Samples (45)
GSM1112445 Young Leaf of hybrid, biological rep1
GSM1112446 Young Leaf of hybrid, biological rep2
GSM1112447 Young Leaf of hybrid, biological rep3
Relations
BioProject PRJNA195934

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45718_RAW.tar 205.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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