|
| Status |
Public on Dec 31, 2013 |
| Title |
Expression data from primary CB erythroblasts, immortalized/induced erythroblasts, Fetal liver CD34+ blood stem cells, adult CD34+ blood stem cells, erythroleukemia cell line (TF-1) and human ESC and iPSCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The supply of red blood cells (RBCs) is not sufficient in many developing countries or in developed countries for patients who need chronic transfusion from best-matched donors. Ex vivo expansion and maturation of human erythroid precursor cells (erythroblasts) could represent a potential solution. Proliferating erythroblasts can be expanded from human umbilical cord blood mononuclear cells (CB MNCs) ex vivo for 10^6-10^7 fold (in ~50 days) before undergoing senescence. Here, we report that ectopic expression of three to four genetic factors that have been used for iPS cell derivation enables CB-derived erythroblasts to undergo extended ex vivo expansion (≥10^51 fold in ~9 months) in a defined suspension culture condition without change of cell identity or function. These vastly expanding erythroblasts maintain homogeneously immature erythroblast phenotypes, a normal diploid karyotype and dependence on specific combination of cytokines and hormone for survival and proliferation throughout the continuous expansion period. When switched to a culture condition for terminal maturation, these immortalized erythroblasts gradually exit cell cycle, decrease cell size, accumulate hemoglobin, condense nuclei and eventually give rise to enucleated hemoglobin-containing erythrocytes. Our result may ultimately lead to the development of unlimited sources of cultured RBCs for optimally-matched or personalized transfusion medicine.
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| Overall design |
We compared the global gene expression profiles of different human cell types: iE: immortalized erythroblasts generated by genetic reprogramming from pCBE; pCBE: primary cord blood-derived erythroblasts; CD34+: CD34+ purified hematopoietic stem/progenitor cells from adult blood or fetal liver; TF-1: a human erythroleukemia cell line; ESC: human embryonic stem cells; iPSCs: human induced pluripotent stem cells. We want to see the relationship among these cell types. We included multiple samples (biological replicates) for most cell types.
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| Contributor(s) |
Huang X, Talbot C Jr, Tsang K |
| Citation(s) |
24002691 |
| |
| Submission date |
Feb 07, 2013 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Xiaosong Huang |
| E-mail(s) |
xhuang18@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Department |
Medicine/Hematology
|
| Lab |
Linzhao Cheng
|
| Street address |
733 North Broadway, Rm780
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
|
| Samples (14)
|
| GSM1079548 |
primary CB-derived erythroblasts at day 19 of expansion culture (donor a) |
| GSM1079549 |
primary CB-derived erythroblasts at day 19 of expansion culture (donor b) |
| GSM1079550 |
primary CB-derived erythroblasts at day 19 of expansion culture (donor b) (subset CD235a+) |
| GSM1079551 |
primary CB-derived erythroblasts at day 19 of expansion culture (donor b) (subset CD235a-) |
| GSM1079552 |
immortalized erythroblasts (donor b) at day 40 of expansion culture (after viral transduction) |
| GSM1079553 |
immortalized erythroblasts (donor b) at day 141 of expansion culture (after viral transduction) |
| GSM1079554 |
Human adult blood isolated CD34+ cells |
| GSM1079555 |
Human fetal liver isolated CD34+ cells |
| GSM1079556 |
Human erythroleukemia cell line TF-1 cultured with Epo for 8 days |
| GSM1079557 |
Human induced pluripotent stem cells derived from adult blood cells |
| GSM1079558 |
Human induced pluripotent stem cells derived from adult blood cells (Large T antigen) |
| GSM1079559 |
Human induced pluripotent stem cells derived from adult mesenchymal stromal cells |
| GSM1079560 |
Human induced pluripotent stem cells derived from mesenchymal stromal cells |
| GSM1079561 |
Human Embryonic stem cells |
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| Relations |
| BioProject |
PRJNA188759 |
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