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Series GSE44136 Query DataSets for GSE44136
Status Public on Dec 31, 2013
Title Expression data from primary CB erythroblasts, immortalized/induced erythroblasts, Fetal liver CD34+ blood stem cells, adult CD34+ blood stem cells, erythroleukemia cell line (TF-1) and human ESC and iPSCs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The supply of red blood cells (RBCs) is not sufficient in many developing countries or in developed countries for patients who need chronic transfusion from best-matched donors. Ex vivo expansion and maturation of human erythroid precursor cells (erythroblasts) could represent a potential solution. Proliferating erythroblasts can be expanded from human umbilical cord blood mononuclear cells (CB MNCs) ex vivo for 10^6-10^7 fold (in ~50 days) before undergoing senescence. Here, we report that ectopic expression of three to four genetic factors that have been used for iPS cell derivation enables CB-derived erythroblasts to undergo extended ex vivo expansion (≥10^51 fold in ~9 months) in a defined suspension culture condition without change of cell identity or function. These vastly expanding erythroblasts maintain homogeneously immature erythroblast phenotypes, a normal diploid karyotype and dependence on specific combination of cytokines and hormone for survival and proliferation throughout the continuous expansion period. When switched to a culture condition for terminal maturation, these immortalized erythroblasts gradually exit cell cycle, decrease cell size, accumulate hemoglobin, condense nuclei and eventually give rise to enucleated hemoglobin-containing erythrocytes. Our result may ultimately lead to the development of unlimited sources of cultured RBCs for optimally-matched or personalized transfusion medicine.
Overall design We compared the global gene expression profiles of different human cell types: iE: immortalized erythroblasts generated by genetic reprogramming from pCBE; pCBE: primary cord blood-derived erythroblasts; CD34+: CD34+ purified hematopoietic stem/progenitor cells from adult blood or fetal liver; TF-1: a human erythroleukemia cell line; ESC: human embryonic stem cells; iPSCs: human induced pluripotent stem cells. We want to see the relationship among these cell types. We included multiple samples (biological replicates) for most cell types.
Contributor(s) Huang X, Talbot C Jr, Tsang K
Citation(s) 24002691
Submission date Feb 07, 2013
Last update date Jan 25, 2019
Contact name Xiaosong Huang
Organization name Johns Hopkins University
Department Medicine/Hematology
Lab Linzhao Cheng
Street address 733 North Broadway, Rm780
City Baltimore
State/province Maryland
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (14)
GSM1079548 primary CB-derived erythroblasts at day 19 of expansion culture (donor a)
GSM1079549 primary CB-derived erythroblasts at day 19 of expansion culture (donor b)
GSM1079550 primary CB-derived erythroblasts at day 19 of expansion culture (donor b) (subset CD235a+)
BioProject PRJNA188759

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Supplementary file Size Download File type/resource
GSE44136_RAW.tar 60.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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