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Series GSE43954 Query DataSets for GSE43954
Status Public on Mar 28, 2013
Title Diet specific expression profiles_MA1
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary Analysis of wildtype (N2) C. elegans fed different diets: E. coli OP50, E. coli HT115 and Comamonas DA1877
Dietary composition has major effects on physiology. Here we show that developmental rate, reproduction and lifespan are altered in C. elegans fed Comamonas DA1877 relative to those fed a standard E. coli OP50 diet. We identify a set of genes that change in expression in response to this diet, and use the promoter of one of these (acdh-1) as a dietary sensor. Remarkably, the effects on transcription and development occur even when Comamonas DA1877 is diluted with another diet, suggesting that Comamonas DA1877 generates a signal that is sensed by the nematode. Surprisingly, the developmental effect is independent from TOR and insulin signaling. Rather, Comamonas DA1877 affects cyclic gene expression during molting, likely through the nuclear hormone receptor NHR-23. Altogether, our findings indicate that different bacteria elicit various responses via distinct mechanisms, which has implications for diseases such as obesity and the interactions between the human microbiome and intestinal cells.
 
Overall design 3 biological replicates for each condition, OP50 is reference sample
 
Contributor(s) MacNeil LT, Watson E, Arda HE, Zhu LJ, Walhout AJ
Citation(s) 23540701
Submission date Jan 31, 2013
Last update date Jul 06, 2016
Contact name Lesley T MacNeil
E-mail(s) lesley.macneil@umassmed.edu
Organization name UMass Medical School
Department Program in Systems Biology
Lab Walhout
Street address 368 Plantation St
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (9)
GSM1074938 N2 young adult on E. coli OP50 rep 1
GSM1074939 N2 young adult on E. coli OP50 rep 2
GSM1074940 N2 young adult on E. coli OP50 rep 3
This SubSeries is part of SuperSeries:
GSE43959 Diet-Induced Developmental Acceleration Independent of TOR and Insulin in C. elegans
Relations
BioProject PRJNA188225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43954_RAW.tar 21.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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