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| Status |
Public on Sep 12, 2013 |
| Title |
The Condition-Dependent Transcriptional Landscape of Burkholderia pseudomallei |
| Platform organism |
Burkholderia pseudomallei K96243 |
| Sample organisms |
Burkholderia pseudomallei; Burkholderia pseudomallei K96243; Burkholderia pseudomallei Bp22 |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Bacterial transcriptomes are dynamic, context-specific and condition-dependent. Infection by the soil bacterium, Burkholderia pseudomallei, causes melioidosis, an often fatal infectious disease of humans and animals. Possessing a large multi-chromosomal genome, B. pseudomallei is able to persist and survive in a multitude of environments. To obtain a comprehensive overview of B. pseudomallei expressed transcripts, we initiated whole-genome transcriptome profiling covering a broad spectrum of conditions and exposures – a so-called “condition compendium”. Using the compendium, we confirmed many previously-annotated genes and operons, and also identified hundreds of novel transcripts including anti-sense transcripts and non-coding RNAs. By systematically examining genes exhibiting highly similar expression patterns, we ascribed putative functions to previously uncharacterized genes, and identified novel regulatory elements controlling these expression patterns. We also used the compendium to elucidate candidate virulence pathways associated with quorum-sensing and infection in mice. Our study showcases the power of a B. pseudomallei condition compendium as a valuable resource for understanding microbial physiology and the pathogenesis of melioidosis.
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| Overall design |
The transcriptome profiles of Bp exposed to 82 conditions were captured using a custom-designed tiling microarray and compiled into a compendium revealing novel genomic features and co-expression network.
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| Contributor(s) |
Ooi W, Ong E |
| Citation(s) |
24068961 |
| |
| Submission date |
Dec 30, 2012 |
| Last update date |
Dec 12, 2013 |
| Contact name |
Wen Fong Ooi |
| E-mail(s) |
ooiwf@gis.a-star.edu.sg
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| Organization name |
Genome Institute of Singapore
|
| Lab |
Infectious Disease 2
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| Street address |
60 Biopolis Street, Genome, #02-01
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| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (1) |
| GPL16432 |
NimbleGen Burkholderia pseudomallei K96243 385K tiling array [071211_BPK96243_CO_Tiling] |
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| Samples (332)
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| Relations |
| BioProject |
PRJNA184926 |
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