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| Status |
Public on Nov 15, 2013 |
| Title |
Suppression of miRNA-708 by polycomb group promotes metastases by calcium-induced cell migration |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The progression of cancer to metastatic disease is a major cause of death. We identified miR-708 being transcriptionally repressed by polycomb repressor complex (PRC2)-induced H3-K27 trimethylation in metastatic breast cancer. miR-708 targets the endoplasmic reticulum protein neuronatin (Nnat) to decrease intracellular calcium (Ca2+) level, resulting in reduction of activation of ERK and FAK, decreased cell migration, and impaired metastases. Functional complementation experiments with Nnat-3’UTR mutant, which is refractory to suppression by miR-708, rescued cell migration and metastasis defects. In breast cancer patients, miR-708 expression was decreased in lymph node and distal metastases, suggesting a metastasis-suppressive role. Our findings uncover a mechanistic role for miR-708 in metastasis and provide a rationale for developing miR-708 as a therapeutic agent against metastatic breast cancer.
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| Overall design |
Sequencing miRNAs from Human breast cancer cells: MCF10A, MCF7, MDA-MB-231, MDA-MB-LM2
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| Contributor(s) |
Ryu S, Mittal V |
| Citation(s) |
23328481 |
| Submission date |
Nov 15, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Dingcheng Gao |
| E-mail(s) |
dig2009@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
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| Department |
CT Surgery
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| Street address |
1300 York Ave
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA181009 |
| SRA |
SRP017190 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42313_miRNA_fold_change.txt.gz |
15.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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