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| Status |
Public on Apr 03, 2013 |
| Title |
Host microRNA molecular signatures associated with human H1N1 and H3N2 influenza A viruses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
In this study, we performed a miRNA global profiling in human lung epithelial cells (A549) infected by two different subtypes of human influenza A viruses (H1N1 and H3N2).
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| Overall design |
A549 cells were either mock-infected or infected at a multiplicity of infection (MOI) of 1 with H1N1 or H3N2 viruses, and total RNAs were isolated at 24 hours post-infection (hpi). An MOI of 1 was performed to ensure that 100% of the cells were infected at 24 hpi, a strategy that we have previously validated and used for a transcriptional profiling study of infected cells (Josset et al. , 2010). The purified RNAs were subjected to reverse transcription using a pool of miRNA RT primers (Human pool A v2.1, Applied Biosystems) and subsequently amplified and quantified by RT-qPCR in a TaqMan array MicroRNA card (Applied biosystems).
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| Contributor(s) |
Terrier O, Textoris J, Carron C, Marcel V, Bourdon J, Rosa-Calatrava M |
| Citation(s) |
23343627 |
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| Submission date |
Nov 02, 2012 |
| Last update date |
Jun 04, 2013 |
| Contact name |
olivier Terrier |
| Organization name |
Université Claude Bernard Lyon 1
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| Department |
Laboratoire de Virologie et Pathologie Humaine
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| Street address |
Rue Guillaume Paradin
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| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
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| Platforms (1) |
| GPL13328 |
TaqMan(r) Array Human MicroRNA A Cards v2.0 |
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| Samples (8)
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| GSM1030532 |
A549, H1N1, 24hpi (rep2) |
| GSM1030533 |
A549, H1N1, 24hpi (rep3) |
| GSM1030534 |
A549, H3N2, 24hpi (rep1) |
| GSM1030535 |
A549, H3N2, 24hpi (rep2) |
| GSM1030536 |
A549, H3N2, 24hpi (rep3) |
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| Relations |
| BioProject |
PRJNA178739 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42003_fold_change.txt.gz |
9.1 Kb |
(ftp)(http) |
TXT |
| GSE42003_non-normalized_data.txt.gz |
10.0 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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