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Series GSE39761 Query DataSets for GSE39761
Status Public on Dec 01, 2013
Title CD8+ T cell gene expression after different antigen dose stimulations
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study aimed to identify the human T cell response toward different antigen doses. Fresh isolated CD8+ T cells from peripheral blood mononuclear cells (PBMCs) were stimulated with influenza M1 peptide-loaded autologous monocyte-derived dendritic cells for 2 weeks. A high M1 peptide antigen dose (10uM on moDC, HD1-5) and an optimal antigen dose (10nM on moDC OD1-5) were used. M1-specific CD8 T cells were tetramer sorted at the end of the 2 weeks. RNA was extracted and analyzed for gene expression using Agilent 8X60K gene expression microarrays.
Overall design 5 independent samples from 3 different HLA A2+ blood donors.
Contributor(s) Chiu YL, Schneck JP
Citation(s) 24292711
Submission date Jul 30, 2012
Last update date Nov 27, 2018
Contact name Yen-Ling Chiu
Organization name Johns Hopkins University
Street address 733 N. Broadway, BRB 615
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (10)
GSM978826 High Dose 1
GSM978827 High Dose 2
GSM978828 High Dose 3
BioProject PRJNA171595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE39761_RAW.tar 126.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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