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| Status |
Public on Dec 01, 2013 |
| Title |
CD8+ T cell gene expression after different antigen dose stimulations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This study aimed to identify the human T cell response toward different antigen doses. Fresh isolated CD8+ T cells from peripheral blood mononuclear cells (PBMCs) were stimulated with influenza M1 peptide-loaded autologous monocyte-derived dendritic cells for 2 weeks. A high M1 peptide antigen dose (10uM on moDC, HD1-5) and an optimal antigen dose (10nM on moDC OD1-5) were used. M1-specific CD8 T cells were tetramer sorted at the end of the 2 weeks. RNA was extracted and analyzed for gene expression using Agilent 8X60K gene expression microarrays.
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| |
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| Overall design |
5 independent samples from 3 different HLA A2+ blood donors.
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| |
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| Contributor(s) |
Chiu YL, Schneck JP |
| Citation(s) |
24292711 |
| Submission date |
Jul 30, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Yen-Ling Chiu |
| Organization name |
Johns Hopkins University
|
| Street address |
733 N. Broadway, BRB 615
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (10)
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| Relations |
| BioProject |
PRJNA171595 |
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