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| Status |
Public on Jul 19, 2012 |
| Title |
Microarray analysis of FXR-regulated genes in murine small intestine. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Obstruction of bile flow results in bacterial proliferation and mucosal injury in the small intestine that can lead to the translocation of bacteria across the epithelial barrier and systemic infection. These adverse effects of biliary obstruction can be inhibited by administration of bile acids. Here we show that the farnesoid X receptor (FXR), a nuclear receptor for bile acids, induces genes involved in enteroprotection and inhibits bacterial overgrowth and mucosal injury in ileum caused by bile duct ligation. Mice lacking FXR have increased ileal levels of bacteria and a compromised epithelial barrier. These findings reveal a central role for FXR in protecting the distal small intestine from bacterial invasion and suggest that FXR agonists may prevent epithelial deterioration and bacterial translocation in patients with impaired bile flow. In this report we have examined the role of FXR in the ileum. We demonstrate that it plays a crucial role in preventing bacterial overgrowth and maintaining the integrity of the intestinal epithelium
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| Overall design |
Mice were administered the FXR ligand GW4064 or vehicle for 14h. The ilium was removed and processed for array analysis. Each sample group was done in duplicate.
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| Contributor(s) |
Inagaki T, Kliewer SA |
| Citation(s) |
16473946 |
| |
| Submission date |
Jul 19, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
sochsner@bcm.edu
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA170996 |
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