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GEO help: Mouse over screen elements for information. |
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Status |
Public on May 23, 2006 |
Title |
Gene Expression Profile Signatures Indicate a Role for Wnt Signaling in Endothelial Commtment From Embryonic Stem Cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Embryonic stem (ES) cells have the potential to generate a variety of cell lineages including endothelial cells, blood cells and smooth muscle cells. flk1-expressing cells derived from ES cells serve as vascular progenitors. We have used global gene expression analysis in order to establish a comprehensive list of candidate genes in the developing vasculature during ES cell differentiation in vitro. A large set of genes, including growth factors, cell surface molecules, transcriptional factors, and members of several signal transduction pathways that are known to be involved in vasculogenesis or angiogenesis, were found to have expression patterns as expected. Some unknown or functionally uncharacterized genes were differentially regulated in flk1+ cells compared with flk1 cells, suggesting possible roles for these genes in vascular commitment. Particularly, multiple components of the Wnt signaling pathway were differentially regulated in flk1+ cells, including Wnt proteins, their receptors, downstream transcriptional factors, and other components belonging to this pathway. Activation of the Wnt signal was able to expand vascular progenitor populations whereas suppression of Wnt activity reduced flk1+ populations. Suppression of Wnt signaling also inhibited the formation of matured vascular capillary-like structures during late stages of EB differentiation. These data indicate a requisite and ongoing role for Wnt activity during vascular development, and the gene expression profiles identify candidate components of this pathway that participate in vascular cell differentiation. Keywords: Time course, development, endothelial cell, angiogenesis, embryonic stem cells, mouse, vasculature, Wnt signaling
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Overall design |
Flk1 positive and negative cells isolated from murine embryoid bodies were subjected to gene expression profiling at 84 hours, 95 hours and eight days post differentiation using the Agilent mouse development platform.
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Contributor(s) |
Wang H, Charles PC, Wu Y, Ren R, Rubin J, Perou C, Bautch V, Patterson C |
Citation(s) |
16601226, 17636018 |
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Submission date |
Dec 06, 2005 |
Last update date |
Jun 06, 2019 |
Contact name |
Monte S Willis |
E-mail(s) |
monte_willis@med.unc.edu
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Phone |
919-843-1938
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Fax |
919-843-4585
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Organization name |
UNC
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Department |
Pathology & Laboratory Medicine
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Lab |
CCBC
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Street address |
103 Mason Farm Road
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City |
Chapel Hill |
State/province |
NC |
ZIP/Postal code |
27759-7525 |
Country |
USA |
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Platforms (1) |
GPL922 |
Agilent-011472 Mouse Development Oligo Microarray G4120A (Feature Number version) |
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Samples (24)
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Relations |
BioProject |
PRJNA93931 |
Supplementary file |
Size |
Download |
File type/resource |
GSE3757_RAW.tar |
820.0 Kb |
(http)(custom) |
TAR |
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