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Status |
Public on Sep 01, 2012 |
Title |
MCF10A cells expressing HER2 and HER3 and grown in three-dimensional cultures |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The tyrosine kinase receptors HER2 and HER3 play an important role in breast cancer. The HER2/HER3 heterodimer is a critical oncogenic unit associated with reduced relapse-free and decreased overall survival. We provide gene expression profile of the mammary epithelial cells MCF10A expressing HER2, HER3 or HER2/HER3 and grown in three-dimensional cultures for 15 days in the presence of heregulin, a known HER3-ligand that stabilizes and activates the HER2/HER3 heterodimer.
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Overall design |
The mammary epithelial cells MCF10A were transduced with retroviral vectors expressing HER2, HER3 or both. Cells from each group were grown for 15 days in three-dimensional cultures. At the end of the experiment, RNA was extracted for gene expression analysis.
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Contributor(s) |
Aceto N, Thiry S, Roloff T, Bentires-Alj M |
Citation(s) |
23062209 |
Submission date |
Apr 03, 2012 |
Last update date |
Jul 26, 2018 |
Contact name |
Tim Roloff |
E-mail(s) |
tim.roloff@fmi.ch
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Organization name |
FMI
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Department |
Functional Genomics
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Street address |
Maulbeerstrasse 66
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City |
Basel |
ZIP/Postal code |
4058 |
Country |
Switzerland |
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Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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Samples (8)
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Relations |
BioProject |
PRJNA157529 |