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Series GSE36338 Query DataSets for GSE36338
Status Public on Mar 27, 2012
Title A Role for Small RNAs in DNA Double-Strand Break Repair
Organisms Arabidopsis thaliana; Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Here we identify a novel class of small RNAs that are ~21-nucleotide in length and are produced from the sequences in the vicinity of DNA double strand break (DSB) sites in Arabidopsis and humans. We named them diRNAs for DSB-induced small RNAs. In Arabidopsis, the biogenesis of diRNAs requires the PI3 kinase ATR, RNA polymerase IV (Pol IV), and Dicer-like proteins. Mutations in these proteins as well as in Pol V cause significant reduction in DSB repair efficiency. diRNAs are recruited by Argonaute 2 (AGO2) to mediate DSB repair. In humans, knocking down Dicer or Ago2 causes a significant reduction in DSB repair. Our findings reveal a novel biological function for small RNAs in the DSB repair pathway. We propose that diRNAs may function as guide molecules for chromatin modifications or recruitment of repair complexes at DSB sites to facilitate repair.
Overall design 28 samples from Arabidopsis thaliana in various genetic backgrounds and 5 samples from the human cells, small RNA sequencing
Contributor(s) Wei W, Ba Z, Wu Y, Qi Y
Citation(s) 22445173
Submission date Mar 07, 2012
Last update date May 15, 2019
Contact name Yijun Qi
Organization name National Institute of Biological Sciences
Lab Yijun Qi's lab
Street address #7 Science Park Road, Zhongguancun Life Science Park
City Beijing
ZIP/Postal code 102206
Country China
Platforms (3)
GPL9062 Illumina Genome Analyzer (Arabidopsis thaliana)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11221 Illumina Genome Analyzer IIx (Arabidopsis thaliana)
Samples (33)
GSM889254 R (DGU.US) line,Col-0 13-day seedlings
GSM889255 T (DSB-triggering) line, Col-0 13-day seedlings
GSM889256 RxT line,Col-0 13-day seedlings
SRA SRP011369
BioProject PRJNA153297

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE36338_RAW.tar 687.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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