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Series GSE35185 Query DataSets for GSE35185
Status Public on Jan 31, 2012
Title Pan-genomic analysis of bovine monocyte-derived macrophage gene expression in response to in vitro infection with Mycobacterium avium subspecies paratuberculosis
Organism Bos taurus
Experiment type Expression profiling by array
Summary Background: Mycobacterium avium subspecies paratuberculosis (MPTb) is the causative agent of Johne’s disease, an intestinal disease of ruminants with major economic consequences. MPTb bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases of infection that can lead to immunopathology and disease dissemination. Consequently, analysis of the macrophage transcriptome in response to MPTb infection can provide valuable insights into the molecular mechanisms that underlie Johne’s disease. Here, we investigate pan-genomic gene expression in bovine monocyte-derived macrophages (MDM) purified from seven age-matched non-related females, in response to in vitro infection with MPTb (multiplicity of infection 2:1) at intervals of 2 hours, 6 hours and 24 hours post-infection.
Results: Differentially expressed genes were identified by comparing the transcriptomes of the infected MDM to the control MDM at each time point (adjusted P-value threshold ≤ 0.10). 1,050 differentially expressed unique genes were identified 2 hours post-infection, with 974 and 84 differentially expressed unique genes detected 6 hours and 24 hours post-infection, respectively. Furthermore, in the infected MDM the number of upregulated genes exceeded the number of downregulated genes at each time point, with the fold-change in expression for the upregulated genes markedly higher than that for the downregulated genes.
Conclusions: Inspection and systems analysis of the differentially expressed genes revealed changes in the expression profiles of genes involved in the inflammatory response, cell signalling pathways and apoptosis. The transcriptional changes associated with cellular signalling and the inflammatory response may reflect different immuno-modulatory mechanisms that underlie host-pathogen interactions during infection.
 
Overall design Affymetrix GeneChip® Bovine Genome Arrays were used to examine gene expression of bovine monocyte-derived macrophages (MDM) after in vitro challenge with Mycobacterium avium subspecies paratuberculosis across a time series of 2 hr, 6 hr and 24 hr post-challenge. A 0 hr control treatment was also generated and seven different age-matched female Holstein-Friesian cattle were used for each time-point/treatment combination for a total of 49 microarrays, one of which was excluded from analysis since it did not pass quality control.
 
Contributor(s) MacHugh DE, Taraktsoglou M, Killick KE, Nalpas NC, Browne JA, Park SD, Hokamp K, Gormley E, Magee DA
Citation(s) 22455317
Submission date Jan 18, 2012
Last update date Dec 17, 2012
Contact name David E MacHugh
E-mail(s) david.machugh@ucd.ie
Phone +353-1-7166256
Organization name University College Dublin
Department College of Agriculture, Food Science and Veterinary Medicine
Lab Animal Genomics Laboratory
Street address University College Dublin, Belfield
City Dublin
ZIP/Postal code D4
Country Ireland
 
Platforms (1)
GPL2112 [Bovine] Affymetrix Bovine Genome Array
Samples (48)
GSM823727 bovine monocyte-derived macrophage non-challenged at T0 rep1
GSM823728 bovine monocyte-derived macrophage non-challenged at T0 rep2
GSM823729 bovine monocyte-derived macrophage non-challenged at T0 rep3
Relations
BioProject PRJNA150677

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35185_RAW.tar 102.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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