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| Status |
Public on Jul 09, 2012 |
| Title |
Affymetrix SNP array data for DNA copy number analysis of supernatant DNA |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
|
| Summary |
DNA contained in the supernatant of cultured malignant cells as well as from plasma/serum of cancer patients is able to transform recipient immortal murine NIH3T3 cells. We demonstrate for the first time that human circulating DNA is able to drive tumor progression in an immunocompetent colon carcinogenesis rat model and that treatment with DNAse I/proteases has antitumor effects in a xenograft nude mice model. DNA copy number analysis of the DNA purifed from the supernatat of SW480 cell cultures was analyzed to determine if specific regions of the genome are represented in this fraction.
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| Overall design |
We hybridized genomic DNA from parental SW480 cells, the DNA isolated from supernatant of SW480 cells, onto the 500K Affymetrix genotyping array set, following the manufacturer´s instructions, and compared their DNA copy number profile against a common reference generated using data from the International HapMap project.
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| Contributor(s) |
Hidalgo-Miranda A, González AD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 12, 2012 |
| Last update date |
May 17, 2017 |
| Contact name |
Alfredo Hidalgo-Miranda |
| E-mail(s) |
ahidalgo@inmegen.gob.mx
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| Phone |
+52-55-53501966
|
| Organization name |
National Institute of Genomic Medicine
|
| Street address |
Periferico Sur 4124, Torre Zafiro II, piso 6
|
| City |
Mexico City |
| ZIP/Postal code |
01900 |
| Country |
Mexico |
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| Platforms (2) |
| GPL3718 |
[Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array |
| GPL3720 |
[Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array |
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| Samples (4)
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| GSM861349 |
DNA from the SW480 cell line [Mapping250K_Nsp] |
| GSM861350 |
DNA obtained from the supernatant of SW480 cell cultures [Mapping250K_Nsp] |
| GSM861351 |
DNA from the SW480 cell line [Mapping250K_Sty] |
| GSM861352 |
DNA obtained from the supernatant of SW480 cell cultures [Mapping250K_Sty] |
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| Relations |
| BioProject |
PRJNA150843 |
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