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| Status |
Public on Dec 22, 2011 |
| Title |
LNGFR- and Thy-1-based prospective isolation of human mesenchymal stem cells reveals functionally distinct subpopulations. |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
Human multipotent mesenchymal stem cells (MSCs), isolated based on their adherence to plastic, show poor growth and differentiation and frequently contain contaminating cells, which limits to clarify their own characteristics. In this study, the identification of two cell surface markers, LNGFR and Thy-1, allowed the prospective isolation of highly purified clonogenic human MSCs. Furthermore, single cell sorting assays followed by in vitro expansion revealed three distinct MSC subpopulations: rapidly-, moderately- and slowly proliferating MSC clones (RPCs, MPCs and SPCs, respectively). While RPCs exhibited robust multilineage differentiation and self-renewal potency, MPCs and SPCs contained a majority of senescent cells and exhibited frequent genome errors.
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| Overall design |
Single cell sorting assays followed by in vitro expansion revealed three distinct MSC subpopulations: rapidly-, moderately- and slowly proliferating MSC clones (RPCs, MPCs and SPCs, respectively).
Sample: RPC, MPC and SPC (n=3).
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| Contributor(s) |
Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Renault-Mihara F, Houlihan DD, Okano H, Matsuzaki Y |
| Citation(s) |
24052950 |
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| Submission date |
Dec 15, 2011 |
| Last update date |
Mar 23, 2015 |
| Contact name |
Yo Mabuchi |
| E-mail(s) |
yomabuchi@2009.jukuin.keio.ac.jp
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| Organization name |
Keio University School of Medicine
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| Department |
Physiology
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| Street address |
35 Shinanomachi, Shinjuku-ku
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| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL10123 |
Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version) |
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| Samples (9)
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| GSM849618 |
rapidly proliferating single cell derived mesenchymal cell from bone marrow 1 |
| GSM849619 |
rapidly proliferating single cell derived mesenchymal cell from bone marrow 2 |
| GSM849620 |
rapidly proliferating single cell derived mesenchymal cell from bone marrow 3 |
| GSM849621 |
moderately proliferating single cell derived mesenchymal cell from bone marrow 1 |
| GSM849646 |
moderately proliferating single cell derived mesenchymal cell from bone marrow 2 |
| GSM849647 |
moderately proliferating single cell derived mesenchymal cell from bone marrow 3 |
| GSM849648 |
slowly proliferating single cell derived mesenchymal cell from bone marrow 1 |
| GSM849688 |
slowly proliferating single cell derived mesenchymal cell from bone marrow 2 |
| GSM849689 |
slowly proliferating single cell derived mesenchymal cell from bone marrow 3 |
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| Relations |
| BioProject |
PRJNA151333 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34484_RAW.tar |
163.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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