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Series GSE34301 Query DataSets for GSE34301
Status Public on Dec 20, 2012
Title Poised RNA Polymerase II changes over developmental time and prepares genes for future expression (ChIP-seq data)
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Poised RNA polymerase II is predominantly found at developmental control genes and is thought to allow their rapid and synchronous induction in response to extracellular signals. How the recruitment of poised RNA Pol II is regulated during development is not known. By isolating muscle tissue from Drosophila embryos at five stages of differentiation, we show that the recruitment of poised Pol II occurs at many genes de novo and this makes them permissive for future gene expression. When compared to other tissues, these changes are stage-specific and not tissue-specific. In contrast, Polycomb group repression is tissue-specific and in combination with Pol II (the balanced state) marks genes with highly dynamic expression. This suggests that poised Pol II is temporally regulated and is held in check in a tissue-specific fashion. We compare our data to mammalian embryonic stem cells and discuss a framework for predicting developmental programs based on chromatin state.
 
Overall design ChIP-seq for Pol II, H3K4me3 and H3K27me3 in various Drosophila embryos and tissues
 
Contributor(s) Gaertner B, Johnston J, Chen K, Zeitlinger J
Citation(s) 23260668
Submission date Dec 09, 2011
Last update date May 15, 2019
Contact name Julia Zeitlinger
E-mail(s) jbz@stowers.org
Organization name Stowers Institute for Medical Research
Lab Zeitlinger Lab
Street address 1000 E 50th St
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
 
Platforms (1)
GPL11203 Illumina Genome Analyzer IIx (Drosophila melanogaster)
Samples (45)
GSM846902 Pol II ChIP-seq from Drosophila neuronal cells, 8WG16 antibody, replicate 1
GSM846903 Pol II ChIP-seq from 6-8 h Drosophila muscle cells, 4H8 antibody, replicate 1
GSM846904 Pol II ChIP-seq from 6-8 h Drosophila muscle cells, 4H8 antibody, replicate 2
This SubSeries is part of SuperSeries:
GSE34304 Poised RNA Polymerase II changes over developmental time and prepares genes for future expression
Relations
SRA SRP009672
BioProject PRJNA156421

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Supplementary file Size Download File type/resource
GSE34301_RAW.tar 4.7 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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