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Series GSE34044 Query DataSets for GSE34044
Status Public on Dec 01, 2011
Title Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Tumors contain a fraction of cancer stem cells that maintain the propagation of the disease. The CD34‏CD38_ cells, isolated from acute myeloid leukemia (AML), were shown to be enriched leukemic stem cells (LSC). We isolated the CD34‏CD38_ cell fraction from AML and compared their gene expression profiles to the CD34‏CD38‏ cell fraction, using microarrays. We found 409 genes that were at least twofold over- or underexpressed between the two cell populations. These include underexpression of DNA repair, signal transduction and cell cycle genes, consistent with the relative quiescence of stem cells, and chromosomal aberrations and mutations of leukemic cells. Comparison of the LSC expression data to that of normal hematopoietic stem cells (HSC) revealed that 34% of the modulated genes are shared by both LSC and HSC, supporting the suggestion that the LSC originated within the HSC progenitors. We focused on the Notch pathway since Jagged-2, a Notch ligand was found to be overexpressed in the LSC samples. We show that DAPT, an inhibitor of gamma-secretase, a protease that is involved in Jagged and Notch signaling, inhibits LSC growth in colony formation assays. Identification of additional genes that regulate LSC self-renewal may provide new targets for therapy.
Overall design Microarrays were used to compare the gene expression patterns between AML CD34+CD38- cells and AML CD34+CD38+
Contributor(s) Gal H, Amariglio N, Trakhtenbrot L, Jacob-Hirsch J, Margalit O, Avigdor A, Nagler A, Tavor S, Ein-Dor L, Lapidot T, Domani E, Rechavi G, Givol D
Citation(s) 17039238
Submission date Nov 30, 2011
Last update date Oct 25, 2022
Contact name jasmine Jacob
Phone 0523790500
Organization name Sheba Medical Center at Tel HaShomer
Street address haChevel 33
City Shoham
State/province -Select-
ZIP/Postal code 60850
Country Israel
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (8)
GSM840928 sorted AML (CD34+CD38-) rep1
GSM840929 sorted AML (CD34+CD38+) rep1
GSM840930 sorted AML (CD34+CD38-) rep2
BioProject PRJNA148063

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Supplementary file Size Download File type/resource
GSE34044_RAW.tar 14.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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