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Sample GSM840933 Query DataSets for GSM840933
Status Public on Dec 01, 2011
Title sorted AML (CD34+CD38+) rep2
Sample type RNA
 
Source name sorted AML (CD34+CD38+)
Organism Homo sapiens
Characteristics cell type: AML (CD34+CD38+)
Treatment protocol Leukemic peripheral blood (PB) samples from five AML patients were collected after obtaining informed consent.Mononuclear cells (MNC) were purified from the samples by Ficoll– Hypaque density gradient centrifugation, resulting in approximately 1–3 108 cells per sample. To determine the CD34 and CD38 content, cells were labeled with anti-human CD34- fluorescein isothiocyanate (FITC) anti-CD38-phycoerythrin (PE) and IgG isotype control monoclonal antibodies (Miltenyi Biotec, Auburn, CA, USA) and the CD34 and CD38 expression levels were determined for each sample using FACS-Calibur (Becton Dickinson, Franklin Lakes, NJ, USA).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 600 ng total RNA (Affymetrix wt_sensetarget_label_manual)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol affymetrix
Data processing MAS5
 
Submission date Nov 30, 2011
Last update date Oct 25, 2022
Contact name jasmine Jacob
E-mail(s) j-jacob@sheba.health.gov.il
Phone 0523790500
Organization name Sheba Medical Center at Tel HaShomer
Street address haChevel 33
City Shoham
State/province -Select-
ZIP/Postal code 60850
Country Israel
 
Platform ID GPL571
Series (1)
GSE34044 Gene expression profiles of AML derived stem cells: similarity to hematopoietic stem cells

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 449 P
AFFX-BioB-M_at 835.5 P
AFFX-BioB-3_at 575.8 P
AFFX-BioC-5_at 1179.3 P
AFFX-BioC-3_at 1414 P
AFFX-BioDn-5_at 2623.2 P
AFFX-BioDn-3_at 4794.8 P
AFFX-CreX-5_at 11238.1 P
AFFX-CreX-3_at 14021.5 P
AFFX-DapX-5_at 2.7 A
AFFX-DapX-M_at 9 A
AFFX-DapX-3_at 2.6 A
AFFX-LysX-5_at 5 A
AFFX-LysX-M_at 2.5 A
AFFX-LysX-3_at 18.1 P
AFFX-PheX-5_at 2.9 A
AFFX-PheX-M_at 1.8 A
AFFX-PheX-3_at 13.4 A
AFFX-ThrX-5_at 0.9 A
AFFX-ThrX-M_at 1.7 A

Total number of rows: 22277

Table truncated, full table size 388 Kbytes.




Supplementary file Size Download File type/resource
GSM840933_H84HGrestain.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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