|
| Status |
Public on Mar 01, 2012 |
| Title |
Identification of miRNA target genes in C. elegans by RIP-chip-SRM |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Other
Expression profiling by array
|
| Summary |
RIP-chip-SRM : a New Combinatorial Large Scale Approach Identifies a Set of Translationally Regulated bantam/miR 58 Targets in C. elegans
RNA binding protein immunopurification + microarray + targeted protein quantification via Selected Reaction Monitoring
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
|
| |
|
| Contributor(s) |
Marko J, Michael HO, Andre GP, Ruedi A, Hubert R |
| Citation(s) |
22454234 |
| |
| Submission date |
Oct 12, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Marko Jovanovic |
| E-mail(s) |
markojov@broadinstitute.org
|
| Organization name |
Broad Institute of MIT and Harvard
|
| Department |
Core Group (Regev group) member
|
| Lab |
Regev lab
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
| GPL14724 |
Washington University/Genome Sequencing Center C. elegans 23K [oligoID version] |
|
| Samples (13)
|
| GSM815845 |
C. elegans, whole animal, mixed-stage, strain N2 (wild type). Replicate 1 |
| GSM815846 |
C. elegans, whole animal, mixed-stage, strain N2 (wild type). Replicate 2 |
| GSM815847 |
C. elegans, whole animal, mixed-stage, strain N2 (wild type). Replicate 3 |
| GSM815848 |
C. elegans, whole animal, mixed-stage, strain WS4303 (alg 1(tm492); opIs205). Replicate 1 |
| GSM815849 |
C. elegans, whole animal, mixed-stage, strain WS4303 (alg 1(tm492); opIs205). Replicate 2 |
| GSM815850 |
C. elegans, whole animal, mixed-stage, strain WS4303 (alg 1(tm492); opIs205). Replicate 3 |
| GSM815851 |
WS5041 (mir 58(n4640); alg 1(tm492); opIs205). Replicate 1 |
| GSM815852 |
WS4303 (alg 1(tm492); opIs205). Replicate 1 |
| GSM815853 |
WS5041 (mir 58(n4640); alg 1(tm492); opIs205). Replicate 2 |
| GSM815854 |
WS4303 (alg 1(tm492); opIs205). Replicate 2 |
| GSM815855 |
WS5041 (mir 58(n4640); alg 1(tm492); opIs205). Replicate 3 |
| GSM815856 |
WS4303 (alg 1(tm492); opIs205). Replicate 3 |
| GSM815857 |
WS5041 total vs. WS4303 total (3 replicates) |
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| This SuperSeries is composed of the following SubSeries: |
| GSE32941 |
Purification of TAP::ALG-1 complexes and associated RNA from mixed-stage TAP::ALG-1 transgenic (WS4303) and wild-type (N2) animals |
| GSE32942 |
Microarray analysis of TAP::ALG-1 associated RNAs isolated from synchronized 'wild-type' animals and 'mir-58' mutants |
| GSE32943 |
Expression analysis of WS4303 (wild type) vs WS5041 (mir-58 mutants) total mRNA |
|
| Relations |
| BioProject |
PRJNA146457 |
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