 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 01, 2012 |
| Title |
Expression data from wildtype and unc-37 mutant A-class motor neurons in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
In Caenorhabditis elegans, VA and VB motor neurons arise as lineal sisters but synapse with different interneurons to regulate locomotion. VA-specific inputs are defined by the UNC-4 homeoprotein and its transcriptional corepressor, UNC-37/Groucho, which function in the VAs to block the creation of chemical synapses and gap junctions with interneurons normally reserved for VBs. To reveal downstream genes that control this choice, we have employed a cell-specific microarray strategy that has now identified unc-4-regulated transcripts. One of these genes, ceh-12, a member of the HB9 family of homeoproteins, is normally restricted to VBs. We show that expression of CEH-12/HB9 in VA motor neurons in unc-4 mutants imposes VB-type inputs. Thus, this work reveals a developmental switch in which motor neuron input is defined by differential expression of transcription factors that select alternative presynaptic partners. The conservation of UNC-4, HB9, and Groucho expression in the vertebrate motor circuit argues that similar mechanisms may regulate synaptic specificity in the spinal cord.
|
| |
|
| Overall design |
We employ the mRNA-tagging method to isolate poly(A) RNA from wildtype and unc-37 mutant A-class motor neurons by expressing a 3X FLAG-tagged poly(A) binding protein PAB-1 in DA/VA neurons under control of the unc-4 promoter. A 2-round IVT protocol (modified from the Affymetrix small-sample protocol) was used to convert starting RNA into biotinylated aRNA.
|
| |
|
| Contributor(s) |
Von Stetina SE, Spencer WC, Miller III DM, Fox RM |
| Citation(s) |
17289921 |
| |
| Submission date |
Sep 29, 2011 |
| Last update date |
Jul 06, 2016 |
| Contact name |
William Clay Spencer |
| E-mail(s) |
clip78@gmail.com
|
| Phone |
4409356643
|
| Organization name |
Vanderbilt University
|
| Department |
Cell & Developmental Biology
|
| Lab |
David Miller
|
| Street address |
465 21st Ave S
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (6)
|
| GSM803570 |
unc-37(e262) A-class, biological rep1 |
| GSM803571 |
unc-37(e262) A-class, biological rep2 |
| GSM803572 |
unc-37(e262) A-class, biological rep3 |
|
| Relations |
| BioProject |
PRJNA147881 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32467_RAW.tar |
19.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...