|
| Status |
Public on Oct 24, 2011 |
| Title |
Mobile 24 nt small RNAs direct transcriptional gene silencing in the root meristems of Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We grafted Transcriptional Gene Silencing (TGS)-inducing wild type Arabidopsis and a mutant that is compromised in 24 nucleotide (nt) small RNA (sRNA) production onto a wild type reporter line. We observed that 21-24 nt sRNAs were transmitted across a graft union yet only the 24 nt sRNAs directed RNA-dependent DNA methylation (RdDM) and TGS of a transgene promoter in meristematic cells.
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| |
|
| Overall design |
Analysis of sRNAs associated with mobile TGS by deep sequencing, biological replicates.
10 unique samples, 5 biological replicates.
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| |
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| Contributor(s) |
Melnyk CW, Molnar A, Bassett A, Baulcombe DC |
| Citation(s) |
21962713 |
| Submission date |
Aug 25, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Krys Kelly |
| Organization name |
University of Cambridge
|
| Department |
Plant Sciences
|
| Lab |
Baulcombe Group
|
| Street address |
Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 3EA |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL9302 |
Illumina Genome Analyzer II (Arabidopsis thaliana) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA145305 |
| SRA |
SRP012589 |
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