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| Status |
Public on Jun 18, 2012 |
| Title |
Gene expression dynamics in yeast BY4741 and a strain lacking MSN2 and MSN4 responding to 0.4mM H2O2 over time (0-60min) |
| Platform organism |
Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae S288C |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
Numerous factors have been implicated in regulating gene expression changes, including changes to nucleosome occupancy. Here we followed dynamic changes to nucleosome occupancy, gene expression and DNA binding of the transcription factor Msn2p genome-wide in yeast cells responding to hydrogen peroxide and reveal new relationships between regulators of stress-dependent gene expression in yeast.
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| Overall design |
Gene expression was measured in response to 0.4mM H2O2 in the S288c derivative BY4741 in wild-type cells and cells lacking MSN2 and MSN4. A single replicate of a time course spanning from 4 to 60 minutes after treatment in each cell type. An additional 3 repliactes were collected from cells 30 minutes after treatment.
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| Contributor(s) |
Huebert DJ, Gasch AP |
| Citation(s) |
22354995 |
| |
| Submission date |
Jul 25, 2011 |
| Last update date |
Jul 07, 2012 |
| Contact name |
Dana J Huebert |
| Organization name |
University of Wisconsin-Madison
|
| Department |
Genetics
|
| Lab |
Audrey Gasch
|
| Street address |
425G Henry Mall
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL9529 |
UW-Madison Saccharomyces cerevisiae 380K tiling array 51mer oligos 2006-10-12 |
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| Samples (22)
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| GSM766197 |
yeast_0.4mM_H2O2_wildtype_30min_rep2 |
| GSM766198 |
yeast_0.4mM_H2O2_wildtype_60min |
| GSM766199 |
yeast_0.4mM_H2O2_wildtype_30min_rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE30901 |
Dynamic changes to nucleosome occupancy and genomic expression in yeast responding to oxidative stress |
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| Relations |
| BioProject |
PRJNA154581 |
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