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| Status |
Public on Dec 25, 2011 |
| Title |
Programming human pluripotent stem cells into adipocytes [Agilent] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The utility of human pluripotent stem cells as a tool for understanding disease and as a renewable source of cells for transplantation therapies is dependent on efficient differentiation protocols that convert these cells into relevant adult cell types. Here we report the robust and efficient differentiation of human pluripotent stem cells into adipocytes. We found that inducible expression of PPARG2 in pluripotent stem cell-derived mesenchymal progenitor cells programmed their development towards an adipocyte cell fate. Using this approach, multiple human pluripotent cell lines were differentiated into adipocytes with efficiencies of 85% to 90%. These pluripotent stem cell-derived adipocytes retained their identity independent of transgene expression, could be maintained in culture for several weeks, expressed mature markers, and exhibited mature functional properties such as lipid catabolism in response to a beta-adrenergic stimulus. Global transcriptional and lipid metabolomic analyses further confirmed the identity and maturity of these pluripotent stem cell-derived adipocytes.
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| Overall design |
Mesenchymal progenitor cells (MPCs) derived from human embryonic stem cells hESCs and induced pluripotent stem cells (iPSCs) along with adipose-derived stromal vascular cells (ADSVCs) were subjected to induction of PPAR2 and compared to primary fat samples. Overall 2 ADSVC (ADSVC 24 nd 49) lines, 1 hESC (HUES9) line and 1 iPSC (BJRiPS) line were differentiated into MPCs, PPAR2 programmed, and compared to untreated MPCs and primary fat samples from 2 individuals. Each condition is either represented in duplicate or triplicate and there are two universal reference spots to aid in slide-dependant batch effects (24 samples total).
Supplementary file(s):
GeneSymbol-collapsed data represent the final normalized data used for analyses in the manuscript.
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| Citation(s) |
22246346 |
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| Submission date |
Jun 16, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Tim D Ahfeldt |
| E-mail(s) |
tim.ahfeldt@mssm.edu
|
| Organization name |
Icahn School of Medicine at Mount Sinai
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| Department |
Neuroscience
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| Lab |
Tim D Ahfeldt
|
| Street address |
1425 Madison Ave
|
| City |
NYC |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE30041 |
Programming human pluripotent stem cells into adipocytes |
|
| Relations |
| BioProject |
PRJNA155149 |
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