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| Status |
Public on Aug 16, 2013 |
| Title |
DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis (smRNA-seq) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) negatively regulate their targets by 1) repressing translation, 2) endonucleolytic RNA cleavage, or 3) DNA methylation resulting in transcriptional silencing. P-body/decapping components are likely required for translational repression, but are not known to function in other posttranscriptional regulatory pathways or to affect smRNA levels. Here, we show that the P-body/decapping protein DCP5 is required for miRNA-mediated translational repression but not cleavage, and to regulate the transcription of specific miRNAs. We find that this protein also affects the abundance of tRNA-derived smRNAs. Significantly, DCP5 is required for the transcriptional silencing and DNA methylation of numerous transposable/repetitive elements and imprinted genes, indicating that it is a novel component of the RNA-directed DNA methylation pathway. Our results demonstrate that DCP5 and likely the P-body itself are required for multiple smRNA-mediated silencing pathways and provide the first evidence for the spatial separation of translational inhibition and cleavage by miRNAs.
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| Overall design |
small RNA (smRNA) expression comparison between wildtype (Col-0) and dcp5 mutant plants in Arabidopsis
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| Contributor(s) |
Zheng Q, Endres MW, Dragomir I, Yang L, Weber R, Silverman IM, Poethig RS, Gregory BD |
| Citation(s) |
23150631 |
| Submission date |
Apr 11, 2011 |
| Last update date |
Jan 05, 2016 |
| Contact name |
Qi Zheng |
| E-mail(s) |
e00011027@gmail.com
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| Phone |
1-215-898-0808
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| Organization name |
University of Pennsylvania
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| Department |
Department of Biology
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| Lab |
Gregory Lab
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| Street address |
433 S. University Ave.
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL9302 |
Illumina Genome Analyzer II (Arabidopsis thaliana) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE28524 |
DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis |
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| Relations |
| BioProject |
PRJNA143051 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28512_RAW.tar |
402.0 Mb |
(http)(custom) |
TAR (of FASTA, TXT) |
| GSE28512_README.txt |
429 b |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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