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| Status |
Public on Aug 22, 2011 |
| Title |
Expression data from Burkholderia multivorans cystic fibrosis clinical isolates |
| Platform organisms |
Burkholderia cenocepacia J2315; Burkholderia multivorans ATCC 17616 |
| Sample organism |
Burkholderia multivorans |
| Experiment type |
Expression profiling by array
|
| Summary |
Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection.
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| Overall design |
For expression profiling, overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in SM medium were diluted to an initial OD640 nm of 0.1 into the same medium. Triplicate samples were cultured at 37ºC with 250 r.p.m. agitation for 17 h and RNA extracted from the two bacterial isolates.
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| Contributor(s) |
Silva IN, Ferreira AS, Becker JD, Moreira LM |
| Citation(s) |
21835880 |
| |
| Submission date |
Apr 01, 2011 |
| Last update date |
May 23, 2012 |
| Contact name |
Leonilde Morais Moreira |
| E-mail(s) |
lmoreira@ist.utl.pt
|
| Phone |
+351 218419031
|
| Organization name |
Instituto Superior Tecnico
|
| Department |
Bioengineering
|
| Lab |
Biological Sciences
|
| Street address |
A. Rovisco Pais
|
| City |
Lisboa |
| ZIP/Postal code |
1049-001 |
| Country |
Portugal |
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| Platforms (1) |
| GPL13356 |
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE30568 |
Burkholderia cepacia complex and cystic fibrosis (CF) patients |
|
| Relations |
| BioProject |
PRJNA153927 |
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