|
| Status |
Public on May 22, 2011 |
| Title |
Two phases of mitogenic signaling unveil roles for p53 and EGR1 in elimination of inconsistent growth signals |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Normal cells require continuous exposure to growth factors, in order to cross a restriction point and commit to cell cycle progression. This can be replaced by two short, appropriately spaced pulses of growth factors, where the first pulse primes a process, which is completed by the second pulse, and enables restriction point crossing. Through integration of comprehensive proteomic and transcriptomic analyses of each pulse, we identified three processes that regulate restriction point crossing: (i) The first pulse induces essential metabolic enzymes and activates p53-dependent restraining processes. (ii) The second pulse eliminates, via the PI3K/AKT pathway, the suppressive action of p53, as well as (iii) sets an ERK-EGR1 threshold mechanism, which digitizes graded external signals into an all-or-none decision obligatory for S-phase entry. Together, our findings uncover novel gating mechanisms, which ensure that cells ignore fortuitous growth factors, and undergo proliferation only in response to consistent mitogenic signals.
|
| |
|
| Overall design |
17 samples; one control sample, time zero.
|
| |
|
| Contributor(s) |
Zwang Y, Drier Y |
| Citation(s) |
21596316 |
| Submission date |
Mar 02, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Yotam Drier |
| E-mail(s) |
yotam.drier@mail.huji.ac.il
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Immunology and Cancer Research
|
| Lab |
Drier
|
| Street address |
Faculty of Medicine, The Hebrew University, Ein Kerem
|
| City |
Jerusalem |
| State/province |
Israel |
| ZIP/Postal code |
9112102 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (17)
|
|
| Relations |
| BioProject |
PRJNA138527 |
Less...
More...