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| Status |
Public on Jun 17, 2024 |
| Title |
Repurposed nuclear speckle and mitosis components induce CTCF clustering to sustain the senescence splicing program [ChIPmentation] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Senescence —the end-point of the replicative life span of normal cells— is characterized by a complex sequence of molecular and biochemical events. One of these is the dramatic reorganization of CTCF into large senescence-induced CTCF clusters (SICCs). However, the molecular determinants, genomic consequences, and functional purpose of SICCs remained unknown. Here, we combine super-resolution imaging, 3D genomics, and functional assays with computational modelling to address all aspects of SICC emergence. First, we used modelling and data from the overexpression of nuclear factors lost upon senescence entry to explain how changes in the nuclear environment allow for CTCF clustering. Second, we discovered that cells entering senescence repurpose SRRM2 —a key component of nuclear speckles— and BANF1 —a protein known to act as ‘molecular glue’ for chromosomes during mitosis— to cluster CTCF on the surface of speckles, while rewiring higher-order genome architecture. Last, we could show that SICCs are required for the faithful execution of the senescence splicing program as their disruption by SRRM2 or BANF1 depletion reverts splicing choices. Together, the senescence-specific clustering of CTCF on nuclear speckles is a new paradigm of a change in the biochemical environment of the nucleus being translated into its architectural reorganization to allow for the deployment of a splicing program driving homeostatic deregulation.
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| Overall design |
We performed ChIPmentation of the Nuclear Speckle component SON and the transcription factor HMGB2, in order to indentify the interaction with CTCF as well as their contribution towards genome architecture and gene expression.
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| Contributor(s) |
Papantonis A, Ramasamy SS, Varamogianni Mamatsi V |
| Citation missing |
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| Submission date |
Jun 14, 2024 |
| Last update date |
Jun 18, 2024 |
| Contact name |
Argyris Papantonis |
| E-mail(s) |
argyris.papantonis@med.uni-goettingen.de
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| Organization name |
University Medical Center Göttingen
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| Department |
Institute of Pathology
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| Street address |
Robert-Koch-Str. 40
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| City |
Göttingen |
| ZIP/Postal code |
37075 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA1124024 |
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