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| Status |
Public on May 29, 2024 |
| Title |
10x single-cell RNAseq profiling of influenza Virus infected Human Lung Tissue Explants (HLTE) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging.
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| Overall design |
Human Lung tissue explant from COPD patients were cultrured in in vitro . These tissue pieces were infected with influenz A virus for 24 hours . After infection, tissue was digested into single cell suspension.
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| Contributor(s) |
Pessler F, Geffers R, Sohail A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 29, 2024 |
| Last update date |
May 30, 2024 |
| Contact name |
Frank Pessler |
| E-mail(s) |
frank.pessler@helmholtz-hzi.de
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| Phone |
0511-220027-167
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| Organization name |
TWINCORE Centre for Experimental and Clinical Infection Reearch
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| Department |
Experimental Infection Research
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| Lab |
Research Group Biomarkers for Infectious Diseases
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| Street address |
Feodor-Lynen-Str. 7
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| City |
Hannover |
| State/province |
Lower Saxony |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (2) |
| GSM8294047 |
Human lung tissue cells uninfected control |
| GSM8294048 |
Human lung tissue cells IAV infected |
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| Relations |
| BioProject |
PRJNA1117768 |
