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| Status |
Public on Mar 24, 2011 |
| Title |
miR-1245 induce genomic instability |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
This experiment is design to evaluate genomic alteration following stably expression of miR-1245, stably knock down of BRCA2 is setup as a positive control
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| Overall design |
Primary normal breast epithelial cells (NBEC) were collected from the mammoplasty material of a 30-year-old woman at the Department of Plastic Surgery, the First Affiliated Hospital of Sun Yat-sen University (P. R. China), in accordance with rules and regulations concerning ethical issues on research use of human subjects in China, and were cultured in the Keratinocyte serum-free medium (Invitrogen, Carlsbad, CA) supplemented with epithelial growth factor, bovine pituitary extract and antibiotics (120 µg/mL streptomycin and 120 µg/mL penicillin). NBEC were infected with virus produced from retro vector pMSCV-miR1245 or empty pMSCV vector; or pSuper Retro BRCA2 shRNA, or pSuper Retro empty vector. After 2 days infection and 3 days culture, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGENE, Valencia, CA). CGH microarray hybridization, data generation and normalization were performed in Shanghai Biochip Corporation.
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| Contributor(s) |
Ying Z, Li J |
| Citation(s) |
22158906 |
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| Submission date |
Jan 19, 2011 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Mengfeng Li |
| E-mail(s) |
limf@mail.sysu.edu.cn
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| Organization name |
Sun Yat-sen University
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| Department |
Zhongshan school of medicine
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| Lab |
Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
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| Street address |
74# zhongshan 2 Rd.
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
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| Platforms (1) |
| GPL10123 |
Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA136359 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26715_RAW.tar |
37.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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