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Series GSE26715 Query DataSets for GSE26715
Status Public on Mar 24, 2011
Title miR-1245 induce genomic instability
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary This experiment is design to evaluate genomic alteration following stably expression of miR-1245, stably knock down of BRCA2 is setup as a positive control
Overall design Primary normal breast epithelial cells (NBEC) were collected from the mammoplasty material of a 30-year-old woman at the Department of Plastic Surgery, the First Affiliated Hospital of Sun Yat-sen University (P. R. China), in accordance with rules and regulations concerning ethical issues on research use of human subjects in China, and were cultured in the Keratinocyte serum-free medium (Invitrogen, Carlsbad, CA) supplemented with epithelial growth factor, bovine pituitary extract and antibiotics (120 µg/mL streptomycin and 120 µg/mL penicillin). NBEC were infected with virus produced from retro vector pMSCV-miR1245 or empty pMSCV vector; or pSuper Retro BRCA2 shRNA, or pSuper Retro empty vector. After 2 days infection and 3 days culture, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGENE, Valencia, CA). CGH microarray hybridization, data generation and normalization were performed in Shanghai Biochip Corporation.
Contributor(s) Ying Z, Li J
Citation(s) 22158906
Submission date Jan 19, 2011
Last update date Dec 06, 2012
Contact name Mengfeng Li
Organization name Sun Yat-sen University
Department Zhongshan school of medicine
Lab Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
Street address 74# zhongshan 2 Rd.
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
Platforms (1)
GPL10123 Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version)
Samples (2)
GSM657845 NBEC miR-1245
BioProject PRJNA136359

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26715_RAW.tar 37.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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