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Series GSE26411 Query DataSets for GSE26411
Status Public on May 27, 2011
Title Interactions with Fibroblasts are Distinct in Basal-like and Luminal Breast Cancers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Basal-like breast cancers have several well-characterized distinguishing molecular features, but most of these are features of the cancer cells themselves. The unique stromal-epithelial interactions, and more generally, microenvironmental features of basal-like breast cancers, have not been well characterized. To identify characteristic microenvironment features of basal-like breast cancer, we performed cocultures of several basal-like breast cancer cell lines with fibroblasts (reduction mammoplasty-derived fibroblast line; RMF) and compared these to cocultures of luminal breast cancer cell lines with fibroblasts. Interactions between basal-like cancer cells and fibroblasts induced expression of numerous interleukins and chemokines, including IL-6, IL-8, CXCL1, CXCL3, and TGFbeta. Under the influence of fibroblasts, basal-like breast cancer cell lines also showed increased migration in vitro. Migration was less pronounced for luminal lines, but these lines were more likely to have altered proliferation. These differences were relevant to tumor biology in vivo, as the gene set that distinguished luminal and basal-like stromal interactions in coculture also distinguishes basal-like from luminal tumors with 98% accuracy in 10-fold CV and 100% accuracy in an independent test set. However, comparisons between cocultures where cells were in direct contact and cocultures where interaction was solely through soluble factors suggest that there is an important impact of direct cell-to-cell contact. The phenotypes and gene expression changes invoked by cancer cell interactions with fibroblasts support the microenvironment and cell-cell interactions as intrinsic features of breast cancer subtypes.
Overall design 99 samples were hybridized with Stratagene common reference and profiled on Agilent microarrays.
Contributor(s) Camp J, Troester MA
Citation(s) 21131600, 25465802
Submission date Jan 03, 2011
Last update date Feb 22, 2018
Contact name Melissa Troester
Organization name University of North Carolina at Chapel Hill
Department Epidemiology
Street address 135 Dauer Drive, CB 7435
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platforms (2)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
GPL8269 UNC PerouLab 244K Custom Human Array version 5
Samples (99)
GSM608138 C_RMF:MDA-MB-231(0:1,48h)
GSM608168 C_RMF:MDA-MB-231(1:0,48h)
GSM608169 C_RMF:MDA-MB-231(1:1,48h)
BioProject PRJNA136873

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Supplementary file Size Download File type/resource
GSE26411_RAW.tar 6.9 Mb (http)(custom) TAR
Processed data included within Sample table

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