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| Status |
Public on Apr 01, 2024 |
| Title |
RNA-seq analysis of young adult fed 5mM tryptophan and control worms |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-seq analysis was used to identify the gene expression changes of young adults after feeding 5mM tryptophan for 36 hours from the L4 period. Based on these sequencing data, the genes that were up-regulated or down-regulated by at least 2 times (|log2 (folding change) | ≥ 1 and padj ≤ 0.05) were analyzed. The analysis of these differentially expressed genes' pathway and gene ontology (GO) enrichment shows that the influence of different genders after feeding 5mM tryptophan is enriched to some signal pathways, including longevity、ribosome and protein degradation pathway in endoplasmic reticulum, etc.
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| Overall design |
When tryptophan was fed, mRNA profile changes for each group were generated by deep sequencing. Use Illumina Gallx
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| Contributor(s) |
zheng s |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 27, 2024 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Shanqing Zheng |
| E-mail(s) |
zhengshanqing@henu.edu.cn
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| Phone |
+8637122892869
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| Organization name |
Henan university
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| Street address |
1 Jinming Avenue North, Longting District, School of Basic Medical Sciences, Henan University
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| City |
Kaifeng |
| ZIP/Postal code |
475004 |
| Country |
China |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (6)
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| GSM8172209 |
Caenorhabditis elegans,20℃, 36 hours after L4 period, rep1 |
| GSM8172210 |
Caenorhabditis elegans,20℃, 36 hours after L4 period, rep2 |
| GSM8172211 |
Caenorhabditis elegans,20℃, 36 hours after L4 period, rep3 |
| GSM8172212 |
Caenorhabditis elegans,20℃, after feeding 5mM tryptophan for 36 hours in L4 period, rep1 |
| GSM8172213 |
Caenorhabditis elegans,20℃, after feeding 5mM tryptophan for 36 hours in L4 period, rep2 |
| GSM8172214 |
Caenorhabditis elegans,20℃, after feeding 5mM tryptophan for 36 hours in L4 period, rep3 |
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| Relations |
| BioProject |
PRJNA1092623 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE262600_RAW.tar |
1.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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