 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 26, 2011 |
| Title |
Transcriptional profiling of C. elegans DAF-19 uncovers a ciliary base-associated protein and a CDK/CCRK/LF2p-related kinase required for intraflagellar transport. |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
Cilia are ubiquitous cell surface projections that modulate various sensory- and motility based processes and are implicated in a growing number of multi-organ genetic disorders termed ciliopathies. As new components required for cilium biogenesis and function remain unidentified, we sought to further define and validate the transcriptional targets of the ciliogenic C. elegans RFX transcription factor DAF-19. To this end, transcriptional profiling of daf-19 mutants (which do not form cilia) and wild-type animals was performed using selectively staged embryos where ciliogenesis occurs in most ciliated sensory neurons
(CSNs). Statistical comparisons between the two populations revealed 881 differentially regulated genes with 1.5-fold change or greater. A subset of these was confirmed by quantitative RT-PCR. Transgenic worms expressing transcriptional-GFP fusions revealed CSN-specific expression patterns for 9 of 12 candidate genes. We show that two uncharacterized candidate genes, which we term dyf-17 and dyf-18 because their
corresponding mutants display dye-filling (Dyf) defects, are important for ciliogenesis. DYF-17 localizes at the base of cilia and interestingly, is specifically required for building the distal segment of sensory cilia. DYF-18 is an evolutionarily conserved CDK-7/CCRK-related serine-threonine kinase that is necessary for the proper function of intraflagellar transport (IFT), a process critical for cilium biogenesis. Together, our comparative microarray study identifies targets of the evolutionarily conserved RFX transcription factor, DAF-19, providing a rich dataset from which to uncover—in addition to DYF-17 and DYF-18—cellular components important for cilium formation and function.
|
| |
|
| Overall design |
4 daf-19,daf12; 4 daf-12; 4 WT
|
| |
|
| Contributor(s) |
Phirke P, Efimenko E, Mohan S, Burghoorn J, Crona F, Bakhoum MW, Trieb M, Schuske K, Jorgensen EM, Piasecki BP, Leroux MR, Swoboda P |
| Citation(s) |
21740898 |
| |
| Submission date |
Nov 28, 2010 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Peter Swoboda |
| E-mail(s) |
peter.swoboda@ki.se
|
| URL |
http://www.bionut.ki.se/groups/psw/index.htm
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of Biosciences and Nutrition
|
| Street address |
Hälsovägen 7
|
| City |
Huddinge |
| State/province |
Stockholm |
| ZIP/Postal code |
141 83 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA133943 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE25633_RAW.tar |
26.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...