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| Status |
Public on Nov 20, 2010 |
| Title |
AMPK and calcineurin induced longevity is mediated by CRTC-1 and CREB |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
AMPK (AAK-2) and calcineurin (TAX-6) mediate longevity exclusively through post-translational modification of CRTC-1, the sole C. elegans CRTC (CREB regulated transcriptional coactivator).
We performed microarrays to examine the transcriptional responses elicited by the pro-longevity: activation of AMPK, deactivation of calcineurin, and decrease of CREB (CRH-1) activity.
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| Overall design |
Gene expression profiles for crh1 (nn3315) and tax-6 (ok2065) mutants, aak-2c (aa1-321) overexpressers and WT (N2) controls were obtained by measuring RNA levels in replicate pools of 3000 synchronized L4 worms. Three replicate pools of each strain were prepared on separate days.
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| Contributor(s) |
Mair W, Morantte I, Rodrigues AP, Manning G, Montminy M, Shaw RJ, Dillin A |
| Citation(s) |
21331044 |
| |
| Submission date |
Nov 19, 2010 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Ana PC Rodrigues |
| E-mail(s) |
arodrigues@salk.edu
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| Organization name |
Salk Institute for Biological Studies
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| Street address |
10010 North Torrey Pines Road
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA133763 |
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