|
| Status |
Public on Jul 08, 2011 |
| Title |
Quantitative analysis of thyroid response in zebrafish embryos |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
In the further study we have employed the Zebrafish gene expression microarray (v2) as a discovery platform to analysis the trancriptome of 5dpf (days post fertilization) embryos exposed from the 2dpf to 5dpf to nanomolar concrations of thyoid hormone. Only less than 5% genes were affected by the treatment. Gene ontology (GO) analyses show that these genes are involved in the haematopoietic system, visual function and skeletal system. Representatives of each GO functional groups were selected and quantified by real-time PCR to validate the microarray data and to use them as a quantitative biomarkers of thyroid receptor activator.
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| Overall design |
The gene expression in Zebrafish was measured in 5 dpf's embryos under 72h Thyroid Hormone treatment at 50nM.
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| Contributor(s) |
Pelayo S, Oliveira E, Thienpont B, Babin P, Raldua D, Piña B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 29, 2010 |
| Last update date |
Apr 06, 2012 |
| Contact name |
Sergi Pelayo Martinez |
| E-mail(s) |
spmbmc@cid.csic.es
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| Organization name |
CSIC
|
| Department |
IDAEA
|
| Lab |
502
|
| Street address |
Jordi Girona n.18
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08034 |
| Country |
Spain |
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| Platforms (1) |
| GPL7301 |
Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA131189 |
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