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| Status |
Public on May 31, 2023 |
| Title |
Transcriptome profiling Zebrafish Sap130a gene mutations in embryogenesis |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
This study profiled the transcriptome of zebrafish embryos harboring mutation in the Sin3A associated Protein 130a (sap130a) gene at 36 hours post fertilization (hpf). The sap130a mutant was generated via Cas9 mediated gene editing. Zebrafish sap130a mutants are viable presumable due to the presence of maternal RNA. Generation of maternal zygotic (MZ) mutants resulted in an incomplete penetrance of heart ventricle defects that were visible as early as 36 hpf. In this experiment the MZsap130a mutant embryos were further sorted for embryos with smaller ventricle (SV) vs normal size ventricle (NV). To extend on this study and to specifically analyze the heart, sap130a mutant and WT hearts at 48 hpf were extracted for RNA-seq analysis.
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| Overall design |
Total RNA was extracted from embryos at 36 hours post fertilization from wildetype, MZsap130a mutants.
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| Contributor(s) |
DeMoya R, Tsang M |
| Citation(s) |
37711853 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL142788 |
Mechanism of LV Hypoplasia in Hypoplastic Left Heart Syndrome |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Michael WaiKok Tsang |
| R01 HL156398 |
Understanding the molecular mechanism of cardiomyocyte dedifferentiation and proliferation during regeneration |
UNIVERSITY OF PITTSBURGH AT PITTSBURGH |
Michael WaiKok Tsang |
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| |
| Submission date |
Mar 23, 2023 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Michael Tsang |
| E-mail(s) |
tsang@pitt.edu
|
| Phone |
4126483248
|
| Organization name |
University of Pittsburgh
|
| Department |
Cell Biology
|
| Street address |
3501 Fifth Ave. BST3-5062
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE228451 |
Transcriptome profiling Zebrafish Sap130a and Sap130b gene mutations in embryogenesis |
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| Relations |
| BioProject |
PRJNA947958 |
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