|
| Status |
Public on Jan 25, 2023 |
| Title |
Characterization and transcriptome analysis of maize small kernel mutant smk7a in different development stages |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The kernel serves as a storage organ for various nutrients and determines the yield and quality of maize. Understanding the mechanisms regulating kernel development is important for maize production. In this study, a small kernel mutant smk7a of maize was characterized. Cytological observation suggested that the development of the endosperm and embryo was arrested in smk7a in the early development stage. Biochemical tests revealed that the starch, zein protein, and indole-3-acetic acid (IAA) contents were significantly lower in smk7a compared with wild-type (WT). Consistent with the defective development phenotype, transcriptome analysis of the kernels 12 and 20 days after pollination (DAP) revealed that the starch, zein, and auxin biosynthesis related genes were dramatically downregulated in smk7a. Genetic mapping indicated that the mutant was controlled by a recessive gene located on chromosome 2. Our results suggest that disrupted nutrition accumulation and auxin synthesis cause the defective endosperm and embryo development of smk7a.
|
| |
|
| Overall design |
We conducted RNA sequencing using the kernels of WT and smk7a mutant collected at 12 and 20 DAP.
|
| |
|
| Contributor(s) |
Wang J, Hu X |
| Citation(s) |
36679067 |
| Submission date |
Dec 22, 2022 |
| Last update date |
Jan 25, 2023 |
| Contact name |
xiaojiao Hu |
| E-mail(s) |
huxiaojiao@caas.cn
|
| Organization name |
Institute of crop science, Chinese academy of agricultural science
|
| Street address |
No.12 Zhongguancun South Main Street, Haidian District
|
| City |
beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA915053 |
Less...
More...