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| Status |
Public on Jun 02, 2011 |
| Title |
The MALAT-1 noncoding RNA Regulates Cell Mobility by Modulating Gene Expressions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MALAT-1, originally identified as a metastasis associated long noncoding transcript in lung adenocarcinoma, is localized to nuclear speckles. However, functional role of MALAT-1 in metastatic characters in cancer cells has not been elucidated. Our current research has revealed that suppression of MALAT-1 expression in lung adenocarcinoma cell line by RNA interference significantly inhibited cellular mobility both in wound healing assay and in migration assay using Boyden's chamber. Moreover, we have identified metastasis/mobility-associated genes among down-regulated genes in the MALAT-1 knockdown cells by DNA microarray analysis. Quantitative analysis of pre-mRNAs has shown that the pre-mRNAs of some genes were suppressed in MALAT-1 knockdown cells but the others were not suppressed even though their matured mRNAs were found to be down-regulated. Our results suggest that MALAT-1 facilitates cell mobility by modulating the expression of metastasis/mobility-associated genes at the transcriptional and post-transcriptional stages.
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| Overall design |
Comparison between control (A549) and knockdown (MALAT-1) cells was performed. Two biological replicates were used, and each replicate was dye-swaped.
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| Contributor(s) |
Miyagawa R, Mizuno R, Nakamura Y, Kenichi I, Rakwal R, Shibaot J, Masuo Y, Hirose T, Akimitsu N |
| Citation(s) |
20937273 |
| |
| Submission date |
Jun 01, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
|
| Phone |
+81-(0)90-1853-7875
|
| Organization name |
University of Tsukuba
|
| Department |
Institute of Health and Sport Sciences
|
| Lab |
Global Sport Innovation (GSI) 403
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| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| GSM549066 |
A549 MALAT-1 knockdown replication 1 |
| GSM549067 |
A549 MALAT-1 knockdown replication 1-1 |
| GSM549068 |
A549 MALAT-1 knockdown replication 2 |
| GSM549069 |
A549 MALAT-1 knockdown replication 2-1 |
|
| Relations |
| BioProject |
PRJNA128933 |
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