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| Status |
Public on May 19, 2023 |
| Title |
Atoh1b-dependent genes in early zebrafish inner ear development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Identification of genes regulated by the distal-less homeodomain transcription factor Atoh1b. To this aim, we performed gene expression profiling analysis using data obtained from RNA-seq of 3 samples each from wt control as well as atoh1b knockdown.
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| Overall design |
Generation of a transcriptome dataset of control and Atoh1b-depleted zebrafish embryos using the pax8:DsRed transgene, which labels the developing OEPD and kidney anlagen. At OEPD stages, we performed fluorescence-activated cell sorting (FACS) and the isolated transcripts from sorted cells of control and Atoh1b-depleted embryos were used for transcriptome profiling. Comparison of control versus Atoh1b-depleted samples reveals numerous differentially expressed genes.
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| Web link |
https://journals.biologists.com/bio/article/12/6/bio059911/313578/Transcriptome-analysis-reveals-an-Atoh1b-dependent
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| Contributor(s) |
Ezhkova D, Schwarzer S, Spieß S, Geffarth M, Machate A, Zöller D, Stucke J, Alexopoulou D, Lesche M, Dahl A, Hans S |
| Citation(s) |
37272628 |
| |
| Submission date |
Dec 08, 2022 |
| Last update date |
Aug 18, 2023 |
| Contact name |
Stefan Hans |
| E-mail(s) |
stefan.hans@tu-dresden.de
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| Organization name |
Technische Universität Dresden
|
| Department |
CRTD - Center for Regenerative Therapies Dresden
|
| Street address |
Fetscherstraße 105
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA910216 |
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