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| Status |
Public on Jan 01, 2023 |
| Title |
The establishment of the anther somatic niche with single cell sequencing |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Maize anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected and their cells isolated using the FX-Cell protocol. scRNA-seq libraries were prepped using the CEL-Seq2 protocol and sequenced with 150bp PE reads with Illumina HiSeq or NovoSeq. The developmental trajectories of the somatic cell layers were investigated.
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| Overall design |
Anthers were dissected from maize plants of the fertile inbred line W23. Anthers ranging in size from 0.5mm to 3.0mm in increments of 0.25mm were dissected, isolated using a Hana Cell Sorter (NamoCell) and used for scRNA-seq. At least two separate W23 plants provided anthers for each stage
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| Contributor(s) |
Marchant DB, Walbot V |
| Citation missing |
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| Submission date |
Nov 30, 2022 |
| Last update date |
Jan 01, 2023 |
| Contact name |
D. Blaine Marchant |
| E-mail(s) |
dbmarchant@gmail.com
|
| Phone |
6507997768
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| Organization name |
Stanford University
|
| Department |
Biology
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| Lab |
Walbot
|
| Street address |
Gilbert Biological Sciences Building, 371 Serra Mall
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (3) |
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| Samples (2182)
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| Relations |
| BioProject |
PRJNA907016 |
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