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Series GSE21 Query DataSets for GSE21
Status Public on Feb 12, 2002
Title snf/swi mutants of S. cerevisiae.
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary The Saccharomyces cerevisiae Snf/Swi complex has been previously demonstrated to control transcription and chromatin structure of particular genes in vivo and to remodel nucleosomes in vitro. We have performed whole-genome expression analysis, using DNA microarrays, to study mutants deleted for a gene encoding one conserved (Snf2) or one unconserved (Swi1) Snf/Swi component. This analysis was performed on cells grown in both rich and minimal media. The microarray results, combined with Northern blot, computational, and genetic analyses, show that snf2Delta and swi1Delta mutations cause similar effects on mRNA levels, that Snf/Swi controls some genes differently in rich and minimal media, and that Snf/Swi control is exerted at the level of individual genes rather than over larger chromosomal domains. In addition, this work shows that Snf/Swi controls mRNA levels of MATalpha-specific genes, likely via controlling transcription of the regulators MATalpha1 and MCM1. Finally, we provide evidence that Snf/Swi acts both as an activator and as a repressor of transcription, and that neither mode of control is an indirect effect of the other.
This study is described in more detail in Sudarsanam P et al.(2000) Proc Natl Acad Sci U S A 97:3364-9
Keywords: other
Contributor(s) Sudarsanam P, Iyer VR, Brown PO, Winston F
Citation(s) 10725359
Submission date Feb 08, 2002
Last update date Jan 18, 2013
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (3)
GPL57 y6k_6400
GPL65 yeast_384_F_v1.0
GPL67 yeast_v1_2.2
Samples (12)
GSM1004 swi1 minimal medium experiment 1
GSM1005 snf2 minimal medium experiment 1
GSM1006 swi1 minimal medium experiment 2
BioProject PRJNA84563

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