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| Status |
Public on Mar 24, 2010 |
| Title |
small RNAs sequences from grafted Arabidopsis thaliana and Nicotiana benthamiana tissues |
| Organisms |
Arabidopsis thaliana; Nicotiana benthamiana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
A silencing signal in plants with an RNA specificity determinant moves through plasmodesmata and the phloem. To identify the mobile RNA we grafted Arabidopsis thaliana shoots to roots that would be a recipient for the silencing signal. Using high throughput sequencing as a sensitive detection method and mutants to block small RNA (sRNA) biogenesis in either source or recipient tissue, we detected endogenous and transgene specific sRNA that moved across the graft union. Surprisingly we found that the mobile endogenous sRNAs account for a substantial proportion of the sRNA in roots and we provide evidence that 24nt mobile sRNAs direct epigenetic modifications in the genome of the recipient cells. Mobile sRNA thus represents a mechanism for transmitting the specification of epigenetic modification and could affect genome defence and responses to external stimuli that have persistent effects in plants.
Keywords: Small RNA Analysis, Epigenetics
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| Overall design |
34 unique samples, 15 Biological Replicates
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| Contributor(s) |
Molnar A, Melnyk CW, Bassett A, Hardcastle TJ, Dunn RM, Baulcombe DC |
| Citation(s) |
20413459 |
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| Submission date |
Mar 05, 2010 |
| Last update date |
Jun 22, 2022 |
| Contact name |
Krys Kelly |
| Organization name |
University of Cambridge
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| Department |
Plant Sciences
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| Lab |
Baulcombe Group
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| Street address |
Downing Street
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3EA |
| Country |
United Kingdom |
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| Platforms (3) |
| GPL9302 |
Illumina Genome Analyzer II (Arabidopsis thaliana) |
| GPL10144 |
Illumina Genome Analyzer (Nicotiana benthamiana) |
| GPL10145 |
Illumina Genome Analyzer II (Nicotiana benthamiana) |
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| Samples (34)
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| GSM518441 |
GxGF-IR/Col rep1 |
| GSM518442 |
GxGF-IR/Col rep2 |
| GSM518443 |
Col/Col rep1 |
| GSM518444 |
Col/Col rep2 |
| GSM518445 |
Col-dcl2,3,4/Col-dcl2,3,4 rep2 |
| GSM518446 |
GxGF-IR/Col shoot rep1 |
| GSM518447 |
GxGF-IR/Col shoot rep2 |
| GSM518448 |
GxA-sde4/Col-dcl2,3,4 rep1 |
| GSM518449 |
GxA-sde4/Col-dcl2,3,4 rep2 |
| GSM518450 |
GxA/Col-dcl2,3,4 rep1 |
| GSM518451 |
GxA/Col-dcl2,3,4 rep2 |
| GSM518452 |
GxA-sde4/Col rep1 |
| GSM518453 |
GxA-sde4/Col rep2 |
| GSM518454 |
GxA/Col rep1 |
| GSM518455 |
GxA/Col rep2 |
| GSM518456 |
GxA-sde4/Col shoot rep1 |
| GSM518457 |
GxA-sde4/Col shoot rep2 |
| GSM518458 |
GxA/Col shoot rep1 |
| GSM518459 |
GxA/Col shoot rep2 |
| GSM518460 |
Col-dcl2,3,4/GxGF-IR rep1 |
| GSM518461 |
Col-dcl2,3,4/GxGF-IR rep2 |
| GSM518462 |
Col/GxGF-IR rep1 |
| GSM518463 |
Col/GxGF-IR rep2 |
| GSM518464 |
GxGF-IR/GFP rep1 |
| GSM518465 |
GxGF-IR/GFP rep2 |
| GSM518466 |
GxGF-IR/GFP shoot rep1 |
| GSM518467 |
GxGF-IR/GFP shoot rep2 |
| GSM518468 |
wt/wt |
| GSM518469 |
wt/GxGF-IR |
| GSM518470 |
GFP/GxGF-IR |
| GSM518471 |
GxGF-IR/GxGF-IR |
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| Relations |
| SRA |
SRP002102 |
| BioProject |
PRJNA124841 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data included within Sample table |
| Raw data are available in SRA |
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