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Series GSE19580 Query DataSets for GSE19580
Status Public on Dec 19, 2009
Title Gene expression in Calu-3 cells after influenza H3N2 and H11N9 infection
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Airway epithelial cells are the initial site of infection with influenza viruses. The innate immune responses of airway epithelial cells to infection have the potential to limit virus replication and induce effective adaptive immune responses. However, relatively little is known about the importance of this innate anti-viral response to infection. Avian influenza viruses are a potential source of future pandemics, therefore it is critical to examine the effectiveness of the host anti-viral system to different influenza viruses. We used a human influenza (H3N2) and a low pathogenic avian influenza (H11N9) to assess and compare the anti-viral responses of bronchial epithelial cells (BECs). After infection, the H3N2 virus replicated more effectively than the H11N9 strain in BECs. This was not due to differential expression of different sialic acid residues on BECs but was attributed to the interference of the host anti-viral responses by H3N2. The H3N2 strain induced a delay in anti-viral signaling and impaired release of type I and type III interferons (IFNs) compared to the H11N9 virus. We then transfected the gene encoding for non-structural (NS) 1 protein into the BECs and the H3N2 NS1 induced a greater inhibition of anti-viral responses compared to the H11N9 NS1. While the low pathogenic avian influenza virus was capable of infecting BECs, the human influenza virus replicated more effectively than avian influenza virus in BECs and this may be at least in part due to a differential ability of the two NS1 proteins to inhibit anti-viral responses. This suggests that the subversion of human anti-viral responses may be an important requirement for influenza viruses to adapt to the human host and induce disease.
 
Overall design One (2hr) or three (6hr, 24hr) replicates of each infection (H3N2, H11N9) and two replicates of media control samples (24hr) were performed and hybridized onto the beadchip.

The supplementary file 'GSE19580.non-normalized.txt' file contains non-normalized data for Samples GSM487974-GSM487989.
 
Contributor(s) Hsu AC, Barr I, Hansbro PM, Wark PA
Citation(s) 20705938
Submission date Dec 18, 2009
Last update date May 14, 2015
Contact name Alan Chen-Yu Hsu
E-mail(s) alan.hsu@studentmail.newcastle.edu.au
Phone +61401784969
Fax +61249855850
Organization name University of Newcastle
Department Respiratory Medicine
Street address Lvl 3 HMRI, John Hunter Hospital, Lookout Rd, New Lambton Height
City Newcastle
State/province NSW
ZIP/Postal code 2305
Country Australia
 
Platforms (1)
GPL8432 Illumina HumanRef-8 WG-DASL v3.0
Samples (16)
GSM487974 Calu-3-H3N2-24h-1
GSM487975 Calu-3-H11N9-02h-1
GSM487976 Calu-3-Media-24h-1
Relations
BioProject PRJNA122351

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19580_ANOVA.txt.gz 202.9 Kb (ftp)(http) TXT
GSE19580_RAW.tar 9.7 Mb (http)(custom) TAR
GSE19580_fold-change.txt.gz 34.8 Kb (ftp)(http) TXT
GSE19580_non-normalized.txt.gz 914.6 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
Processed data provided as supplementary file

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