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Series GSE195618 Query DataSets for GSE195618
Status Public on Mar 01, 2023
Title Systematic comparison of tools used for m6A mapping from nanopore direct RNA sequencing
Organism Mus musculus
Experiment type Other
Summary State-of-the-art algorithms for m6A detection and quantification via nanopore direct RNA sequencing have been continuously developed, little is known about their capacities and limitations, which makes a comprehensive assessment in urgent need. Therefore, we performed comprehensive benchmarking of 10 computational tools relying on current-based and base-calling “errors” strategies for m6A detection by nanopore sequencing.
 
Overall design m6A detection of wild type and Mettl3 knock-out mouse embryonic stem cells.
 
Contributor(s) Zhong Z, Xie Y, Zhang Z, Luo G
Citation(s) 37019930
Submission date Jan 28, 2022
Last update date May 02, 2023
Contact name Zhendong Zhong
E-mail(s) zhongzhd@mail2.sysu.edu.cn
Phone 19806835958
Organization name Sun Yat-sen University
Department School of Life Sciences
Street address Xingangxi Roda 135#
City Guangzhou
State/province Guangdong
ZIP/Postal code 510275
Country China
 
Platforms (1)
GPL24973 MinION (Mus musculus)
Samples (2)
GSM5841801 mES_Nanopore_dRNAseq_WT
GSM5841802 mES_Nanopore_dRNAseq_KO
Relations
BioProject PRJNA801506

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Supplementary file Size Download File type/resource
GSE195618_RAW.tar 3.9 Mb (http)(custom) TAR (of BED)
GSE195618_mES.m6A_DRUMMER.bed.gz 18.7 Kb (ftp)(http) BED
GSE195618_mES.m6A_DiffErr.bed.gz 24.9 Kb (ftp)(http) BED
GSE195618_mES.m6A_Nanocompore.bed.gz 29.0 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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