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Series GSE188968 Query DataSets for GSE188968
Status Public on Dec 20, 2021
Title Geminiviruses employ host DNA glycosylases to subvert DNA methylation-mediated defense
Organisms Arabidopsis thaliana; Nicotiana benthamiana
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation is an epigenetic mechanism that plays important roles in gene regulation and transposon silencing. Active DNA demethylation has evolved to counterbalance DNA methylation at many endogenous loci. Here, we report that active DNA demethylation also targets viral DNAs, tomato yellow leaf curl China virus (TYLCCNV) and its satellite tomato yellow leaf curl China betasatellite (TYLCCNB), to promote their virulence. We demonstrate that the βC1 protein, encoded by TYLCCNB, interacts with a ROS1-like DNA glycosylase in Nicotiana benthamiana and with the DEMETER (DME) DNA glycosylase in Arabidopsis thaliana. The interaction between βC1 and DME facilitates the DNA glycosylase activity to decrease viral DNA methylation and promote viral virulence. These findings reveal that active DNA demethylation can be regulated by a viral protein to subvert DNA methylation-mediated defense.
Overall design To detected methylatuion levels of intergenic region of TYLCCNV and BSCTV, we delvelop a locus specifical bisulfite sequencing in this study.
Contributor(s) Gui X, Liu C, Qi Y, Zhou X
Citation(s) 35102164
Submission date Nov 16, 2021
Last update date Feb 09, 2022
Contact name Yijun Qi
Organization name Tsinghua University
Department School of Life Sciences
Street address NO.1 Qinghuayuan
City Beiing
ZIP/Postal code 100084
Country China
Platforms (2)
GPL23157 HiSeq X Ten (Arabidopsis thaliana)
GPL30965 HiSeq X Ten (Nicotiana benthamiana)
Samples (69)
GSM5692616 pCambia1300/TYLCCNV-IR-Bisulfite-seq_rep1
GSM5692617 pCambia1300/TYLCCNV-IR-Bisulfite-seq_rep2
GSM5692618 pCambia1300/TYLCCNV-IR-Bisulfite-seq_rep3
BioProject PRJNA780919
SRA SRP346400

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Supplementary file Size Download File type/resource
GSE188968_RAW.tar 3.5 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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