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Series GSE183603 Query DataSets for GSE183603
Status Public on Sep 08, 2021
Title Gene expression in commercial human cerebral microvascular endothelial cells (hCMECs) line knock out or not for RNF213
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To study the role of RNF213, we generated RNF213-deficient human cerebral microvascular endothelial cells by the CRISPR-Cas9 double nickase method and showed that RNF213 may be an important regulator of the blood-brain barrier permeability and homeostasis
Overall design One commercial line of hCMECs at passage 33 cultivated in proliferation or at confluency and transfected or not (wild type) with Cas9 without guide RNA (transfection control) or with ZNF213 specific guide RNA (knock-out ZNF213)
Contributor(s) Roy V, Ross J, Pépin R, Cortez Ghio S, Brodeur A, Touzel Deschênes L, Le-Bel G, Phillips D, Milot G, Dion P, Guérin S, Germain L, Berthod F, Auger F, Rouleau G, Dupré N, Gros-Louis F
Citation(s) 34991336
Submission date Sep 07, 2021
Last update date Jan 17, 2022
Contact name Gaëtan Le-Bel
Organization name CUO-Recherche
Lab Sylvain Guérin
Street address Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
City Québec
State/province Quebec
ZIP/Postal code G1S 4L8
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (14)
GSM5563280 Commercial_hCMECs_line_wild_type_confluency_001_rep2
GSM5563281 Commercial_hCMECs_line_transfection_control_confluency_001_rep1
GSM5563282 Commercial_hCMECs_line_transfection_control_confluency_001_rep2
BioProject PRJNA761392

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183603_RAW.tar 178.2 Mb (http)(custom) TAR (of TXT)
GSE183603_processed_data.xlsx 4.6 Mb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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