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| Status |
Public on Sep 08, 2021 |
| Title |
Gene expression in commercial human cerebral microvascular endothelial cells (hCMECs) line knock out or not for RNF213 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To study the role of RNF213, we generated RNF213-deficient human cerebral microvascular endothelial cells by the CRISPR-Cas9 double nickase method and showed that RNF213 may be an important regulator of the blood-brain barrier permeability and homeostasis
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| Overall design |
One commercial line of hCMECs at passage 33 cultivated in proliferation or at confluency and transfected or not (wild type) with Cas9 without guide RNA (transfection control) or with ZNF213 specific guide RNA (knock-out ZNF213)
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| Contributor(s) |
Roy V, Ross J, Pépin R, Cortez Ghio S, Brodeur A, Touzel Deschênes L, Le-Bel G, Phillips D, Milot G, Dion P, Guérin S, Germain L, Berthod F, Auger F, Rouleau G, Dupré N, Gros-Louis F |
| Citation(s) |
34991336 |
| Submission date |
Sep 07, 2021 |
| Last update date |
Jan 17, 2022 |
| Contact name |
Gaëtan Le-Bel |
| E-mail(s) |
gaetan.lebel17@gmail.com
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| Organization name |
CUO-Recherche
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| Lab |
Sylvain Guérin
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| Street address |
Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
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| City |
Québec |
| State/province |
Quebec |
| ZIP/Postal code |
G1S 4L8 |
| Country |
Canada |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (14)
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| GSM5563280 |
Commercial_hCMECs_line_wild_type_confluency_001_rep2 |
| GSM5563281 |
Commercial_hCMECs_line_transfection_control_confluency_001_rep1 |
| GSM5563282 |
Commercial_hCMECs_line_transfection_control_confluency_001_rep2 |
| GSM5563283 |
Commercial_hCMECs_line_knock_out_RNF213_confluency_001_clone1_rep1 |
| GSM5563284 |
Commercial_hCMECs_line_knock_out_RNF213_confluency_001_clone1_rep2 |
| GSM5563285 |
Commercial_hCMECs_line_knock_out_RNF213_confluency_001_clone2_rep1 |
| GSM5563286 |
Commercial_hCMECs_line_knock_out_RNF213_confluency_001_clone2_rep2 |
| GSM5563287 |
Commercial_hCMECs_line_wild_type_proliferation_001_rep1 |
| GSM5563288 |
Commercial_hCMECs_line_wild_type_proliferation_001_rep2 |
| GSM5563289 |
Commercial_hCMECs_line_transfection_control_proliferation_001_rep1 |
| GSM5563290 |
Commercial_hCMECs_line_knock_out_RNF213_proliferation_001_clone1_rep1 |
| GSM5563291 |
Commercial_hCMECs_line_knock_out_RNF213_proliferation_001_clone1_rep2 |
| GSM5563292 |
Commercial_hCMECs_line_knock_out_RNF213_proliferation_001_clone2_rep1 |
| GSM5563293 |
Commercial_hCMECs_line_knock_out_RNF213_proliferation_001_clone2_rep2 |
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| Relations |
| BioProject |
PRJNA761392 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE183603_RAW.tar |
178.2 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE183603_processed_data.xlsx |
4.6 Mb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
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