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| Status |
Public on Jul 11, 2022 |
| Title |
GI is a repressor of ABA transcriptional responses and sensitivity in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional reprogramming plays a key role in drought stress responses, preceding the onset of morphological and physiological adaptations. The best characterised signal regulating gene expression in response to drought is the phytohormone abscisic acid (ABA). ABA-regulated gene expression, biosynthesis and signalling are highly organised in a diurnal cycle, so that ABA-regulated physiological traits occur at the appropriate time of the day. The mechanisms that underpin such diel oscillations in ABA signals are poorly characterised. Here we uncover GIGANTEA (GI) as a key gate keeper of ABA-regulated transcriptional and physiological responses. Time-resolved RNAseq under different irrigation scenarios indicate that gi mutants produce an exaggerated ABA response at midday, despite accumulating wild-type levels ABA. The direct comparison with ABA-deficient mutants confirms the role of GI in controlling ABA-regulated genes and enabled us to distinguish a phase of high ABA sensitivity at dawn, characterized by low GI accumulation.
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| Overall design |
Analysis of RNAsequencing data in different irrigation conditions and genetic backgrounds
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| Contributor(s) |
Siemiatkowska B, Chiara M, Conti L |
| Citation(s) |
35859344 |
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| Submission date |
Jul 29, 2021 |
| Last update date |
Oct 10, 2022 |
| Contact name |
Matteo Chiara |
| E-mail(s) |
matteo.chiara@unimi.it
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| Organization name |
University of Milan
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| Department |
Biosciences
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| Street address |
via celoria 26
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| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
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| Samples (48)
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| GSM5484339 |
Aba1-6, normal watering, time point ZT1 |
| GSM5484340 |
Aba1-6, normal watering, time point ZT1 rep2 |
| GSM5484341 |
Aba1-6 ,drought, time point ZT1 rep1 |
| GSM5484342 |
Aba1-6 ,drought, time point ZT1 rep2 |
| GSM5484343 |
Aba1-6, normal watering, time point ZT8 rep1 |
| GSM5484344 |
Aba1-6, normal watering, time point ZT8 rep2 |
| GSM5484345 |
Aba1-6 ,drought, time point ZT8 rep1 |
| GSM5484346 |
Aba1-6 ,drought, time point ZT8 rep2 |
| GSM5484347 |
Aba1-6, normal watering, time point ZT12 rep1 |
| GSM5484348 |
Aba1-6, normal watering, time point ZT12 rep2 |
| GSM5484349 |
Aba1-6 ,drought, time point ZT12 rep1 |
| GSM5484350 |
Aba1-6 ,drought, time point ZT12 rep2 |
| GSM5484351 |
Aba1-6, normal watering, time point ZT16 rep1 |
| GSM5484352 |
Aba1-6, normal watering, time point ZT16 rep2 |
| GSM5484353 |
Aba1-6 ,drought, time point ZT16 rep1 |
| GSM5484354 |
Aba1-6 ,drought, time point ZT16 rep2 |
| GSM5484355 |
Gi, normal watering, time point ZT1 rep1 |
| GSM5484356 |
Gi, normal watering, time point ZT1 rep2 |
| GSM5484357 |
Gi ,drought, time point ZT1 rep1 |
| GSM5484358 |
Gi ,drought, time point ZT1 rep2 |
| GSM5484359 |
Gi, normal watering, time point ZT8 rep1 |
| GSM5484360 |
Gi, normal watering, time point ZT8 rep2 |
| GSM5484361 |
Gi ,drought, time point ZT8 rep1 |
| GSM5484362 |
Gi ,drought, time point ZT8 rep2 |
| GSM5484363 |
Gi, normal watering, time point ZT12 rep1 |
| GSM5484364 |
Gi, normal watering, time point ZT12 rep2 |
| GSM5484365 |
Gi ,drought, time point ZT12 rep1 |
| GSM5484366 |
Gi ,drought, time point ZT12 rep2 |
| GSM5484367 |
Gi, normal watering, time point ZT16 rep1 |
| GSM5484368 |
Gi, normal watering, time point ZT16 rep2 |
| GSM5484369 |
Gi ,drought, time point ZT16 rep1 |
| GSM5484370 |
Gi ,drought, time point ZT16 rep2 |
| GSM5484371 |
Wt, normal watering, time point ZT1 rep1 |
| GSM5484372 |
Wt, normal watering, time point ZT1 rep2 |
| GSM5484373 |
Wt ,drought, time point ZT1 rep1 |
| GSM5484374 |
Wt ,drought, time point ZT1 rep2 |
| GSM5484375 |
Wt, normal watering, time point ZT8 rep1 |
| GSM5484376 |
Wt, normal watering, time point ZT8 rep2 |
| GSM5484377 |
Wt ,drought, time point ZT 8 rep1 |
| GSM5484378 |
Wt ,drought, time point ZT8 rep2 |
| GSM5484379 |
Wt, normal watering, time point ZT12 rep1 |
| GSM5484380 |
Wt, normal watering, time point ZT12 rep2 |
| GSM5484381 |
Wt ,drought, time point ZT12 rep1 |
| GSM5484382 |
Wt ,drought, time point ZT12 rep2 |
| GSM5484383 |
Wt, normal watering, time point ZT16 rep1 |
| GSM5484384 |
Wt, normal watering, time point ZT16 rep2 |
| GSM5484385 |
Wt ,drought, time point ZT16 rep1 |
| GSM5484386 |
Wt ,drought, time point ZT16 rep2 |
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| Relations |
| BioProject |
PRJNA750649 |
| SRA |
SRP330405 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181083_extended_countsUnique.csv.gz |
1.6 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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