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| Status |
Public on Jul 29, 2021 |
| Title |
Comparison of genes differentially expressed between Medicago R108 and abi3 mutants (NF3185 and NF6003) during different stages of seed development (16, 24, 32 and 40 DAP) |
| Organism |
Medicago truncatula |
| Experiment type |
Expression profiling by array
|
| Summary |
ABI3 is a B3-domain transcription factor that acts as a master regulator of seed maturation. To identify genes that are regulated specifically by ABI3 in the model legume Medicago R108 during seed development, using the Medicago NimbleGen chip, a transcriptomic analysis was performed between abi3 mutants (NF3185 and NF6003) and R108 wild-type at 4 stages of seed development.
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| Overall design |
Two-condition experiment, mutant versus Wt. Biological replicates: 3 control replicates (Wt), 3 treatment replicates (Homozygous mutants) and 4 stages of seed development for NF6003 mutant. 2 control replicates (Wt), 2 treatment replicates (Homozygous mutants) and 3 stages of seed development for NF3185 mutant. Independently grown and harvested. One replicate per array. Dye swap method.
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| Contributor(s) |
Lalanne D, Buitink J, Verdier J |
| Citation(s) |
34451755 |
| |
| Submission date |
Jul 28, 2021 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Julia Buitink |
| E-mail(s) |
julia.buitink@inrae.fr
|
| Phone |
+33241225544
|
| Organization name |
INRAE
|
| Department |
BAP
|
| Lab |
SEED
|
| Street address |
42 rue Georges Morel
|
| City |
Beaucouze |
| ZIP/Postal code |
49070 |
| Country |
France |
| |
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| Platforms (1) |
| GPL16373 |
NimbleGen custom IRHS_Medtr_102K_v1 |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA750411 |
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